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Quantitative Correlation between the Protein Expression Level in Escherichia Coli and Thermodynamic Stability of Protein In Vitro

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Title: Quantitative Correlation between the Protein Expression Level in Escherichia Coli and Thermodynamic Stability of Protein In Vitro
Authors: Wada, Junya Browse this author
Miyazaki, Hiromitsu Browse this author
Kamada, Rui Browse this author
Sakaguchi, Kazuyasu Browse this author →KAKEN DB
Issue Date: Feb-2016
Publisher: Chemical Society of Japan
Journal Title: Chemistry Letters
Volume: 45
Issue: 2
Start Page: 185
End Page: 187
Publisher DOI: 10.1246/cl.151019
Abstract: Protein expression using Escherichia coli is a common and important method for recombinant protein production. Herein, we quantitatively analyzed the correlation between protein expression in vivo and thermodynamic structure stability in vitro using the tetramerization domain of tumor suppressor protein p53. We found a strong positive relationship between the expression level and the thermodynamic stability. Our study suggests that a minimum thermodynamic stability of a protein is required for substantial protein expression in bacterial cells.
Type: article
URI: http://hdl.handle.net/2115/63555
Appears in Collections:理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 坂口 和靖

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