Enhanced production of polyunsaturated fatty acids by enzyme engineering of tandem acyl carrier proteins

Hayashi, Shohei; Satoh, Yasuharu; Ujihara, Tetsuro; Takata, Yusuke; Dairi, Tohru

doi:10.1038/srep35441


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Enhanced production of polyunsaturated fatty acids by enzyme engineering of tandem acyl carrier proteins

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Title:	Enhanced production of polyunsaturated fatty acids by enzyme engineering of tandem acyl carrier proteins
Authors:	Hayashi, Shohei Browse this author
	Satoh, Yasuharu Browse this author →KAKEN DB
	Ujihara, Tetsuro Browse this author
	Takata, Yusuke Browse this author
	Dairi, Tohru Browse this author →KAKEN DB
Issue Date:	19-Oct-2016
Publisher:	Nature Publishing Group
Journal Title:	Scientific reports
Volume:	6
Start Page:	35441
Publisher DOI:	10.1038/srep35441
Abstract:	In some microorganisms, polyunsaturated fatty acids (PUFAs) are biosynthesized by PUFA synthases characterized by tandem acyl carrier proteins (ACPs) in subunit A. These ACPs were previously shown to be important for PUFA productivity. In this study, we examined their function in more detail. PUFA productivities increased depending on the number of ACPs without profile changes in each subunit A of eukaryotic and prokaryotic PUFA synthases. We also constructed derivative enzymes from subunit A with 5 x ACPs. Enzymes possessing one inactive ACP at any position produced similar to 30% PUFAs compared with the parental enzyme but unexpectedly had similar to 250% productivity compared with subunit A with 4 x ACPs. Enzymes constructed by replacing the 3rd ACP with an inactive ACP from another subunit A or ACP-unrelated sequences produced similar to 100% and similar to 3% PUFAs compared with the parental 3rd ACP-inactive enzyme, respectively. These results suggest that both the structure and number of ACP domains are important for PUFA productivity.
Rights:	http://creativecommons.org/licenses/by/4.0/
Type:	article
URI:	http://hdl.handle.net/2115/63752
Appears in Collections:	工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 佐藤康治

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