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Structural, super-resolution microscopy analysis of paraspeckle nuclear body organization

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タイトル: Structural, super-resolution microscopy analysis of paraspeckle nuclear body organization
著者: West, Jason A. 著作を一覧する
Mito, Mari 著作を一覧する
Kurosaka, Satoshi 著作を一覧する
Takumi, Toru 著作を一覧する
Tanegashima, Chiharu 著作を一覧する
Chujo, Takeshi 著作を一覧する
Yanaka, Kaori 著作を一覧する
Kingston, Robert E. 著作を一覧する
Hirose, Tetsuro 著作を一覧する
Bond, Charles 著作を一覧する
Fox, Archa 著作を一覧する
Nakagawa, Shinichi 著作を一覧する
発行日: 2016年 9月27日
出版者: Rockefeller University Press
誌名: Journal of cell biology
巻: 214
号: 7
開始ページ: 817
終了ページ: 830
出版社 DOI: 10.1083/jcb.201601071
抄録: Paraspeckles are nuclear bodies built on the long noncoding RNA Neat1, which regulates a variety of physiological processes including cancer progression and corpus luteum formation. To obtain further insight into the molecular basis of the function of paraspeckles, we performed fine structural analyses of these nuclear bodies using structural illumination microscopy. Notably, paraspeckle proteins are found within different layers along the radially arranged bundles of Neat1 transcripts, forming a characteristic core-shell spheroidal structure. In cells lacking the RNA binding protein Fus, paraspeckle spheroids are disassembled into smaller particles containing Neat1, which are diffusely distributed in the nucleoplasm. Sequencing analysis of RNAs purified from paraspeckles revealed that AG-rich transcripts associate with Neat1, which are distributed along the shell of the paraspeckle spheroids. We propose that paraspeckles sequester core components inside the spheroids, whereas the outer surface associates with other components in the nucleoplasm to fulfill their function.
Rights: ©West et al., 2016. Originally published in The Journal of Cell Biology. doi:10.1083/jcb.201601071
資料タイプ: article
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 中川 真一


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