Title: | Structural, super-resolution microscopy analysis of paraspeckle nuclear body organization |
Authors: | West, Jason A. Browse this author |
Mito, Mari Browse this author |
Kurosaka, Satoshi Browse this author |
Takumi, Toru Browse this author →KAKEN DB |
Tanegashima, Chiharu Browse this author |
Chujo, Takeshi Browse this author |
Yanaka, Kaori Browse this author |
Kingston, Robert E. Browse this author |
Hirose, Tetsuro Browse this author →KAKEN DB |
Bond, Charles Browse this author |
Fox, Archa Browse this author |
Nakagawa, Shinichi Browse this author →KAKEN DB |
Issue Date: | 27-Sep-2016 |
Publisher: | Rockefeller University Press |
Journal Title: | Journal of cell biology |
Volume: | 214 |
Issue: | 7 |
Start Page: | 817 |
End Page: | 830 |
Publisher DOI: | 10.1083/jcb.201601071 |
Abstract: | Paraspeckles are nuclear bodies built on the long noncoding RNA Neat1, which regulates a variety of physiological processes including cancer progression and corpus luteum formation. To obtain further insight into the molecular basis of the function of paraspeckles, we performed fine structural analyses of these nuclear bodies using structural illumination microscopy. Notably, paraspeckle proteins are found within different layers along the radially arranged bundles of Neat1 transcripts, forming a characteristic core-shell spheroidal structure. In cells lacking the RNA binding protein Fus, paraspeckle spheroids are disassembled into smaller particles containing Neat1, which are diffusely distributed in the nucleoplasm. Sequencing analysis of RNAs purified from paraspeckles revealed that AG-rich transcripts associate with Neat1, which are distributed along the shell of the paraspeckle spheroids. We propose that paraspeckles sequester core components inside the spheroids, whereas the outer surface associates with other components in the nucleoplasm to fulfill their function. |
Rights: | ©West et al., 2016. Originally published in The Journal of Cell Biology. doi:10.1083/jcb.201601071 |
Type: | article |
URI: | http://hdl.handle.net/2115/63798 |
Appears in Collections: | 薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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