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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Engineering / Faculty of Engineering >
Peer-reviewed Journal Articles, etc >
Rapid Detection of Cat Cystatin C (cCys-C) Using Immuno-Pillar Chips
| Title: | Rapid Detection of Cat Cystatin C (cCys-C) Using Immuno-Pillar Chips |
| Authors: | Mohammadi, Saeed Browse this author | | Busa, Lori Shayne Alamo Browse this author | | Maeki, Masatoshi Browse this author | | Mohamadi, Reza M. Browse this author | | Ishida, Akihiko Browse this author | | Tani, Hirofumi Browse this author | | Tokeshi, Manabu Browse this author →KAKEN DB |
| Keywords: | Chronic kidney disease | | immunoassay | | cystatin C | | immuno-pillar chips |
| Issue Date: | Dec-2016 |
| Publisher: | 日本分析化学会 |
| Journal Title: | Analytical sciences |
| Volume: | 32 |
| Issue: | 12 |
| Start Page: | 1359 |
| End Page: | 1362 |
| Publisher DOI: | 10.2116/analsci.32.1359 |
| PMID: | 27941268 |
| Abstract: | We demonstrated a rapid immunoassay for detection of cat cystatin C (cCys-C) which is an important marker for chronic kidney disease in cats, using immuno-pillar chips. The required amount of reagent solution is 200 times smaller than that for the conventional ELISA in the 96-well microplate (0.5 mu L versus 100 mu L). In addition, the total assay time in the proposed method is more than 12 times shorter than in the conventional method (20 min versus 240 min). The limit of detection in the new method of 3 ng mL(-1) is comparable to that of the conventional method (1 ng mL(-1)) and it is in the clinically relevant range. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/64585 |
| Appears in Collections: | 工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 渡慶次 学
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