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Rapid Detection of Cat Cystatin C (cCys-C) Using Immuno-Pillar Chips

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Title: Rapid Detection of Cat Cystatin C (cCys-C) Using Immuno-Pillar Chips
Authors: Mohammadi, Saeed Browse this author
Busa, Lori Shayne Alamo Browse this author
Maeki, Masatoshi Browse this author
Mohamadi, Reza M. Browse this author
Ishida, Akihiko Browse this author
Tani, Hirofumi Browse this author
Tokeshi, Manabu Browse this author →KAKEN DB
Keywords: Chronic kidney disease
cystatin C
immuno-pillar chips
Issue Date: Dec-2016
Publisher: 日本分析化学会
Journal Title: Analytical sciences
Volume: 32
Issue: 12
Start Page: 1359
End Page: 1362
Publisher DOI: 10.2116/analsci.32.1359
PMID: 27941268
Abstract: We demonstrated a rapid immunoassay for detection of cat cystatin C (cCys-C) which is an important marker for chronic kidney disease in cats, using immuno-pillar chips. The required amount of reagent solution is 200 times smaller than that for the conventional ELISA in the 96-well microplate (0.5 mu L versus 100 mu L). In addition, the total assay time in the proposed method is more than 12 times shorter than in the conventional method (20 min versus 240 min). The limit of detection in the new method of 3 ng mL(-1) is comparable to that of the conventional method (1 ng mL(-1)) and it is in the clinically relevant range.
Type: article
Appears in Collections:工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 渡慶次 学

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