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A competitive immunoassay system for microfluidic paper-based analytical detection of small size molecules

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Title: A competitive immunoassay system for microfluidic paper-based analytical detection of small size molecules
Authors: Alamo Busa, Lori Shayne Browse this author
Mohammadi, Saeed Browse this author
Maeki, Masatoshi Browse this author
Ishida, Akihiko Browse this author
Tani, Hirofumi Browse this author
Tokeshi, Manabu Browse this author →KAKEN DB
Issue Date: 2016
Publisher: Royal Society of Chemistry
Journal Title: Analyst
Volume: 141
Issue: 24
Start Page: 6598
End Page: 6603
Publisher DOI: 10.1039/c6an01475e
PMID: 27858015
Abstract: The development of a competitive immunoassay system for colorimetric detection on microfluidic paper-based analytical devices (mu PADs) is reported. The mu PADs were fabricated via photolithography to define hydrophilic flow channels and consisted of three main elements: the control and test zones, where target detection was performed, the sample introduction zone, and the competitive capture zone located between the sample introduction zone and the test zone. The chromogenic substrate 3,3', 5,5'-tetra-methylbenzidine (TMB) was deposited at the control and test zones. mu PAD surface modification was performed at the capture zone first via chitosan activation, then the BSA-conjugated target compound was immobilized. The sample solution consisting of the target compound, the peroxidase-conjugated antibody, and the hydrogen peroxide oxidizing agent was introduced into the device and competition occurred at the capture zone, allowing only the target-bound peroxidase-conjugated antibody to travel past the capture zone and into the test zone via capillary action. The developed competitive immunoassay system was successfully demonstrated on the mu PAD detection of biotin as a model compound with a detection limit of 0.10 mu g mL(-1). The applicability of the proposed immunoassay system for point-of-need testing was further demonstrated using aflatoxin B-1, a highly toxic foodborne substance, with a detection limit of 1.31 ng mL(-1). The mu PAD with the competitive immunoassay format showed promising results for practical applications in point-of-need testing involving small molecular weight targets in food and water safety and quality monitoring, environmental analysis, and clinical diagnostics.
Type: article
Appears in Collections:工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 渡慶次 学

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