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Reconstitution of biosynthetic machinery of fungal polyketides : unexpected oxidations of biosynthetic intermediates by expression host
| Title: | Reconstitution of biosynthetic machinery of fungal polyketides : unexpected oxidations of biosynthetic intermediates by expression host |
| Authors: | Fujii, Ryuya Browse this author | | Ugai, Takahiro Browse this author | | Ichinose, Hirofumi Browse this author | | Hatakeyama, Mayumi Browse this author | | Kosaki, Takuto Browse this author | | Gomi, Katsuya Browse this author | | Fujii, Isao Browse this author | | Minami, Atsushi Browse this author | | Oikawa, Hideaki Browse this author →KAKEN DB |
| Keywords: | biosynthesis | | heterologous expression | | Aspergillus oryzae | | solanapyrone | | cytochalasin |
| Issue Date: | 4-Mar-2016 |
| Publisher: | Taylor & Francis |
| Journal Title: | Bioscience biotechnology and biochemistry |
| Volume: | 80 |
| Issue: | 3 |
| Start Page: | 426 |
| End Page: | 431 |
| Publisher DOI: | 10.1080/09168451.2015.1104234 |
| PMID: | 26539980 |
| Abstract: | Reconstitution of whole biosynthetic genes in Aspergillus oryzae has successfully applied for total biosynthesis of various fungal natural products. Heterologous production of fungal metabolites sometimes suffers unexpected side reactions by host enzymes. In the studies on fungal polyketides solanapyrone and cytochalasin, unexpected oxidations of terminal olefin of biosynthetic intermediates were found to give one and four by-products by host enzymes of the transformants harboring biosynthetic genes. In this paper, we reported structure determination of by-products and described a simple solution to avoid the undesired reaction by introducing the downstream gene in the heterologous production of solanapyrone C. |
| Rights: | This is an Accepted Manuscript of an article published by Taylor & Francis in Bioscience biotechnology and biochemistry
on 2016.3.4, available online: http://wwww.tandfonline.com/DOI:10.1080/09168451.2015.1104234. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/64655 |
| Appears in Collections: | 理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 及川 英秋
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