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Reconstitution of biosynthetic machinery of fungal polyketides : unexpected oxidations of biosynthetic intermediates by expression host

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Title: Reconstitution of biosynthetic machinery of fungal polyketides : unexpected oxidations of biosynthetic intermediates by expression host
Authors: Fujii, Ryuya Browse this author
Ugai, Takahiro Browse this author
Ichinose, Hirofumi Browse this author
Hatakeyama, Mayumi Browse this author
Kosaki, Takuto Browse this author
Gomi, Katsuya Browse this author
Fujii, Isao Browse this author
Minami, Atsushi Browse this author
Oikawa, Hideaki Browse this author →KAKEN DB
Keywords: biosynthesis
heterologous expression
Aspergillus oryzae
solanapyrone
cytochalasin
Issue Date: 4-Mar-2016
Publisher: Taylor & Francis
Journal Title: Bioscience biotechnology and biochemistry
Volume: 80
Issue: 3
Start Page: 426
End Page: 431
Publisher DOI: 10.1080/09168451.2015.1104234
PMID: 26539980
Abstract: Reconstitution of whole biosynthetic genes in Aspergillus oryzae has successfully applied for total biosynthesis of various fungal natural products. Heterologous production of fungal metabolites sometimes suffers unexpected side reactions by host enzymes. In the studies on fungal polyketides solanapyrone and cytochalasin, unexpected oxidations of terminal olefin of biosynthetic intermediates were found to give one and four by-products by host enzymes of the transformants harboring biosynthetic genes. In this paper, we reported structure determination of by-products and described a simple solution to avoid the undesired reaction by introducing the downstream gene in the heterologous production of solanapyrone C.
Rights: This is an Accepted Manuscript of an article published by Taylor & Francis in Bioscience biotechnology and biochemistry on 2016.3.4, available online: http://wwww.tandfonline.com/DOI:10.1080/09168451.2015.1104234.
Type: article
URI: http://hdl.handle.net/2115/64655
Appears in Collections:理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 及川 英秋

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