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Requirement for nuclear autoantigenic sperm protein mRNA expression in bovine preimplantation development

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Title: Requirement for nuclear autoantigenic sperm protein mRNA expression in bovine preimplantation development
Authors: Nagatomo, Hiroaki Browse this author
Kohri, Nanami Browse this author
Akizawa, Hiroki Browse this author
Hoshino, Yumi Browse this author
Yamauchi, Nobuhiko Browse this author
Kono, Tomohiro Browse this author
Takahashi, Masashi Browse this author →KAKEN DB
Kawahara, Manabu Browse this author →KAKEN DB
Keywords: bovine embryo
Issue Date: Mar-2016
Publisher: Wiley-Blackwell
Journal Title: Animal science journal
Volume: 87
Issue: 3
Start Page: 457
End Page: 461
Publisher DOI: 10.1111/asj.12538
PMID: 26690724
Abstract: Nuclear autoantigenic sperm protein (NASP) is associated with DNA replication, cell proliferation, and cell cycle progression through its specific binding to histones. The aim of this study was to examine the roles of NASP in bovine preimplantation embryonic development. Using NASP gene knockdown (KD), we confirmed the reduction of NASP messenger RNA (mRNA) expression during preimplantation development. NASP KD did not affect cleavage but significantly decreased development of embryos into the blastocyst stage. Furthermore, blastocyst hatching was significantly decreased in NASP KD embryos. Cell numbers in the inner cell mass of NASP KD blastocysts were also decreased compared to those of controls. These results suggest that NASP mRNA expression is required for preimplantation development into the blastocyst stage in cattle.
Rights: This is the peer reviewed version of the following article:Animal Science Journal, Volume 87(3),pages 457–461, March 2016, which has been published in final form at This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
Type: article (author version)
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 川原 学

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