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Hokkaido University Collection of Scholarly and Academic Papers >
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Peer-reviewed Journal Articles, etc >
Requirement for nuclear autoantigenic sperm protein mRNA expression in bovine preimplantation development
| Title: | Requirement for nuclear autoantigenic sperm protein mRNA expression in bovine preimplantation development |
| Authors: | Nagatomo, Hiroaki Browse this author | | Kohri, Nanami Browse this author | | Akizawa, Hiroki Browse this author | | Hoshino, Yumi Browse this author | | Yamauchi, Nobuhiko Browse this author | | Kono, Tomohiro Browse this author | | Takahashi, Masashi Browse this author →KAKEN DB | | Kawahara, Manabu Browse this author →KAKEN DB |
| Keywords: | bovine embryo | | knockdown | | NASP |
| Issue Date: | Mar-2016 |
| Publisher: | Wiley-Blackwell |
| Journal Title: | Animal science journal |
| Volume: | 87 |
| Issue: | 3 |
| Start Page: | 457 |
| End Page: | 461 |
| Publisher DOI: | 10.1111/asj.12538 |
| PMID: | 26690724 |
| Abstract: | Nuclear autoantigenic sperm protein (NASP) is associated with DNA replication, cell proliferation, and cell cycle progression through its specific binding to histones. The aim of this study was to examine the roles of NASP in bovine preimplantation embryonic development. Using NASP gene knockdown (KD), we confirmed the reduction of NASP messenger RNA (mRNA) expression during preimplantation development. NASP KD did not affect cleavage but significantly decreased development of embryos into the blastocyst stage. Furthermore, blastocyst hatching was significantly decreased in NASP KD embryos. Cell numbers in the inner cell mass of NASP KD blastocysts were also decreased compared to those of controls. These results suggest that NASP mRNA expression is required for preimplantation development into the blastocyst stage in cattle. |
| Rights: | This is the peer reviewed version of the following article:Animal Science Journal, Volume 87(3),pages 457–461, March 2016, which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1111/asj.12538/abstract. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/64658 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 川原 学
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