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Isolation and expression of an elongation-dependent gene of mung bean (Vigna radiata) hypocotyl
| Title: | Isolation and expression of an elongation-dependent gene of mung bean (Vigna radiata) hypocotyl |
| Authors: | Hashimoto, Hiroshi Browse this author | | Yamamoto, Kotaro T. Browse this author →KAKEN DB |
| Issue Date: | Jun-1999 |
| Publisher: | Wiley-Blackwell |
| Journal Title: | Physiologia Plantarum |
| Volume: | 106 |
| Issue: | 2 |
| Start Page: | 224 |
| End Page: | 231 |
| Publisher DOI: | 10.1034/j.1399-3054.1999.106212.x |
| Abstract: | A cDNA clone of an auxin up-regulated gene, ARG8, was isolated from hypocotyl sections of etiolated mung bean [Vigna radiata (L.) Wilczek] seedlings by differential screening. The deduced amino acid sequence suggested that ARG8 may encode a cell wall protein. The steady state mRNA level of ARG8 increased by treatment of hypocotyl sections not only with indole-3-acetic acid (IAA) but also with fusicoccin, and the auxin inducibility was inhibited by the addition of 0.3 M mannitol in the incubation medium. This indicated that it was not auxin but elongation that regulated the expression of ARG8. The promoter activity of the 5′-flanking region of ARG8 was determined by assaying the transient expression of a luciferase fusion gene that was introduced into mung bean hypocotyl sections by the particle bombardment technique. The basal activity of the ARG8 upstream region was about a few tenths of that of a modified cauliflower mosaic virus 35S promoter, and it was increased a few fold by treatment with IAA. The auxin inducibility was completely suppressed by the addition of mannitol. A 5′-deletion analysis showed that a 53-bp region in the ARG8 promoter was important for the basal and elongation-dependent promoter activities. |
| Rights: | This is the peer reviewed version of the following article: Isolation and expression of an elongation-dependent gene of mung bean (Vigna radiata) hypocotyl, which has been published in final form at [10.1034/j.1399-3054.1999.106212.x. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/64932 |
| Appears in Collections: | 理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 山本 興太朗
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