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Pathologic Roles of Receptor-Associated Prorenin System in Idiopathic Epiretinal Membrane

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/65771

Title: Pathologic Roles of Receptor-Associated Prorenin System in Idiopathic Epiretinal Membrane
Authors: Dong, Yoko Browse this author
Kanda, Atsuhiro Browse this author →KAKEN DB
Noda, Kousuke Browse this author →KAKEN DB
Saito, Wataru Browse this author →KAKEN DB
Ishida, Susumu Browse this author →KAKEN DB
Issue Date: 9-Mar-2017
Publisher: Nature Publishing Group
Journal Title: Scientific reports
Volume: 7
Start Page: 44266
Publisher DOI: 10.1038/srep44266
Abstract: Receptor-associated prorenin system (RAPS) refers to the pathogenic mechanism whereby prorenin binding to (pro)renin receptor [(P)RR] dually activates tissue renin-angiotensin system (RAS) and RAS-independent signaling via (P)RR. The aim of this study is to determine the association of RAPS with idiopathic epiretinal membrane (iERM). Reverse transcription-PCR indicated the expression of RAPS components, including (P)RR and Ang II type 1 receptor (AT1R), in iERM tissues and human Müller glial cell line. Double-labeling analyses demonstrated that (P)RR and AT1R were detected in cells positive for glial fibrillary acidic protein, a marker for glial cells, and co-localized with prorenin and angiotensinogen, respectively. Administration of prorenin to Müller glial cells enhanced mRNA expression of fibroblast growth factor 2, while Ang II application stimulated the expression of glial cell line-derived neurotrophic factor, nerve growth factor, and transforming growth factor-β1. These expression levels induced by prorenin or Ang II were reversed by (P)RR or AT1R blockade, respectively. Immunofluorescence revealed tissue co-localization of (P)RR and AT1R with the products of the upregulated genes in vitro. The present findings suggest the involvement of RAPS in the pathogenesis of iERM.
Rights: http://creativecommons.org/licenses/by/4.0/
Type: article
URI: http://hdl.handle.net/2115/65771
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 神田 敦宏

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