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Insertion and Deletion Mismatches Distant from the Target Position Improve Gene Correction with a Tailed Duplex
| Title: | Insertion and Deletion Mismatches Distant from the Target Position Improve Gene Correction with a Tailed Duplex |
| Authors: | Kamiya, Hiroyuki Browse this author →KAKEN DB | | Nishigaki, Natsuki Browse this author | | Ikeda, Akihiro Browse this author | | Yukawa, Seiya Browse this author | | Morita, Yukiko Browse this author | | Nakatsu, Yoshimichi Browse this author →KAKEN DB | | Tsuzuki, Teruhisa Browse this author →KAKEN DB | | Harashima, Hideyoshi Browse this author →KAKEN DB |
| Keywords: | Indel mismatch | | gene correction | | nucleotide sequence conversion | | rpsL gene | | tailed duplex |
| Issue Date: | 3-Jul-2017 |
| Publisher: | Taylor & Francis |
| Journal Title: | Nucleosides, nucleotides & nucleic acids |
| Volume: | 35 |
| Issue: | 7 |
| Start Page: | 379 |
| End Page: | 388 |
| Publisher DOI: | 10.1080/15257770.2016.1163384 |
| PMID: | 27253876 |
| Abstract: | A 5-tailed duplex (TD) DNA corrects a base-substitution mutation. In this study, the effects of insertion and deletion (indel) mismatches distant from the target position on the gene correction were examined. Three target plasmid DNAs with and without indel mismatches approximate to 330 bases distant from the correction target position were prepared, and introduced into HeLa cells together with the TD. The indel mismatches improved the gene correction efficiency and specificity without sequence conversions at the indel mismatch site. These results suggested that the gene correction efficiency and specificity are increased when an appropriate second mismatch is introduced into the TD fragment. |
| Rights: | This is an Accepted Manuscript of an article published by Taylor & Francis in Nucleosides, Nucleotides and Nucleic Acids on 2016, available online: http://www.tandfonline.com/10.1080/15257770.2016.1163384 |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/66349 |
| Appears in Collections: | 薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 紙谷 浩之
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