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Insertion and Deletion Mismatches Distant from the Target Position Improve Gene Correction with a Tailed Duplex

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Title: Insertion and Deletion Mismatches Distant from the Target Position Improve Gene Correction with a Tailed Duplex
Authors: Kamiya, Hiroyuki Browse this author →KAKEN DB
Nishigaki, Natsuki Browse this author
Ikeda, Akihiro Browse this author
Yukawa, Seiya Browse this author
Morita, Yukiko Browse this author
Nakatsu, Yoshimichi Browse this author →KAKEN DB
Tsuzuki, Teruhisa Browse this author →KAKEN DB
Harashima, Hideyoshi Browse this author →KAKEN DB
Keywords: Indel mismatch
gene correction
nucleotide sequence conversion
rpsL gene
tailed duplex
Issue Date: 3-Jul-2017
Publisher: Taylor & Francis
Journal Title: Nucleosides, nucleotides & nucleic acids
Volume: 35
Issue: 7
Start Page: 379
End Page: 388
Publisher DOI: 10.1080/15257770.2016.1163384
PMID: 27253876
Abstract: A 5-tailed duplex (TD) DNA corrects a base-substitution mutation. In this study, the effects of insertion and deletion (indel) mismatches distant from the target position on the gene correction were examined. Three target plasmid DNAs with and without indel mismatches approximate to 330 bases distant from the correction target position were prepared, and introduced into HeLa cells together with the TD. The indel mismatches improved the gene correction efficiency and specificity without sequence conversions at the indel mismatch site. These results suggested that the gene correction efficiency and specificity are increased when an appropriate second mismatch is introduced into the TD fragment.
Rights: This is an Accepted Manuscript of an article published by Taylor & Francis in Nucleosides, Nucleotides and Nucleic Acids on 2016, available online:
Type: article (author version)
Appears in Collections:薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 紙谷 浩之

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