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Digital confocal microscopy using a virtual 4f-system based on numerical beam propagation for depth measurement without mechanical scanning
Title: | Digital confocal microscopy using a virtual 4f-system based on numerical beam propagation for depth measurement without mechanical scanning |
Authors: | Goto, Yuta Browse this author | Okamoto, Atsushi Browse this author →KAKEN DB | Toda, Masataka Browse this author | Kuno, Yasuyuki Browse this author | Nozawa, Jin Browse this author | Ogawa, Kazuhisa Browse this author | Tomita, Akihisa Browse this author →KAKEN DB |
Issue Date: | Aug-2016 |
Publisher: | IOP Publishing |
Journal Title: | Japanese Journal of Applied Physics (JJAP) |
Volume: | 55 |
Issue: | 8 |
Start Page: | 08RE04 |
Publisher DOI: | 10.7567/JJAP.55.08RE04 |
Abstract: | We propose a digital confocal microscope using a virtual 4f-system based on numerical beam propagation for depth measurement without mechanical scanning. In our technique, the information in the sample target along the depth direction is obtained by defocusing the virtual 4f-system, which consists of two virtual lenses arranged in a computer simulation. The principle of our technique is completely different from that of the mechanical scanning method used in the conventional confocal microscope based on digital holography. By using the virtual 4f-system, the measurement and exposure time can be markedly reduced because multilayered tomographic images are generated using a single measurement. In this study, we tested the virtual depth imaging technique by measuring cover glasses arranged along the depth direction. (C) 2016 The Japan Society of Applied Physics |
Rights: | © 2016 The Japan Society of Applied Physics |
Type: | article (author version) |
URI: | http://hdl.handle.net/2115/66896 |
Appears in Collections: | 情報科学院・情報科学研究院 (Graduate School of Information Science and Technology / Faculty of Information Science and Technology) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 後藤 優太
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