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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Agriculture / Faculty of Agriculture >
Peer-reviewed Journal Articles, etc >
Practical Preparation of Epilactose Produced with Cellobiose 2-Epimerase from Ruminococcus albus NE1
| Title: | Practical Preparation of Epilactose Produced with Cellobiose 2-Epimerase from Ruminococcus albus NE1 |
| Authors: | Saburi, Wataru Browse this author →KAKEN DB | | Yamamoto, Takeshi Browse this author | | Taguchi, Hidenori Browse this author | | Hamada, Shigeki Browse this author | | Matsui, Hirokazu Browse this author →KAKEN DB |
| Keywords: | epilactose | | cellobiose 2-epimerase | | practical preparation |
| Issue Date: | Aug-2010 |
| Publisher: | Taylor & Francis |
| Journal Title: | Bioscience, Biotechnology, and Biochemistry |
| Volume: | 74 |
| Issue: | 8 |
| Start Page: | 1736 |
| End Page: | 1737 |
| Publisher DOI: | 10.1271/bbb.100353 |
| PMID: | 20699553 |
| Abstract: | A practical purification method for a non-digestible disaccharide, epilactose (4-O-beta-galactosyl-D-mannose), was established. Epilactose was synthesized from lactose with cellobiose 2-epimerase and purified by the following procedure: (i) removal of lactose by crystallization, (ii) hydrolysis of lactose by beta-galactosidase, (iii) digestion of monosaccharides by yeast, and (iv) column chromatography with Na-form cation exchange resin. Epilactose of 91.1% purity was recovered at 42.5% yield. |
| Rights: | This is an Accepted Manuscript of an article published by Taylor & Francis in Bioscience biotechnology and biochemistry on 2010 Aug, available online: http://wwww.tandfonline.com/10.1271/bbb.100353 |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/67552 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 佐分利 亘
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