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Practical Preparation of Epilactose Produced with Cellobiose 2-Epimerase from Ruminococcus albus NE1

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Title: Practical Preparation of Epilactose Produced with Cellobiose 2-Epimerase from Ruminococcus albus NE1
Authors: Saburi, Wataru Browse this author →KAKEN DB
Yamamoto, Takeshi Browse this author
Taguchi, Hidenori Browse this author
Hamada, Shigeki Browse this author
Matsui, Hirokazu Browse this author →KAKEN DB
Keywords: epilactose
cellobiose 2-epimerase
practical preparation
Issue Date: Aug-2010
Publisher: Taylor & Francis
Journal Title: Bioscience, Biotechnology, and Biochemistry
Volume: 74
Issue: 8
Start Page: 1736
End Page: 1737
Publisher DOI: 10.1271/bbb.100353
PMID: 20699553
Abstract: A practical purification method for a non-digestible disaccharide, epilactose (4-O-beta-galactosyl-D-mannose), was established. Epilactose was synthesized from lactose with cellobiose 2-epimerase and purified by the following procedure: (i) removal of lactose by crystallization, (ii) hydrolysis of lactose by beta-galactosidase, (iii) digestion of monosaccharides by yeast, and (iv) column chromatography with Na-form cation exchange resin. Epilactose of 91.1% purity was recovered at 42.5% yield.
Rights: This is an Accepted Manuscript of an article published by Taylor & Francis in Bioscience biotechnology and biochemistry on 2010 Aug, available online: http://wwww.tandfonline.com/10.1271/bbb.100353
Type: article (author version)
URI: http://hdl.handle.net/2115/67552
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 佐分利 亘

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