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Enantioseparation of hydroxyeicosatetraenoic acids by hydroxypropyl-gamma-cyclodextrin-modified micellar electrokinetic chromatography

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/67771

Title: Enantioseparation of hydroxyeicosatetraenoic acids by hydroxypropyl-gamma-cyclodextrin-modified micellar electrokinetic chromatography
Authors: Kodama, Shuji Browse this author
Nakajima, Shota Browse this author
Ozaki, Hiromichi Browse this author
Takemoto, Ryota Browse this author
Itabashi, Yutaka Browse this author →KAKEN DB
Kuksis, Arnis Browse this author
Keywords: Enantioseparation
Gracilaria
Hydroxyeicosatetraenoic acid
Hydroxypropyl-gamma-cyclodextrin
Micellar electrokinetic chromatography
Issue Date: Dec-2016
Publisher: Wiley-Blackwell
Journal Title: Electrophoresis
Volume: 37
Issue: 23-24
Start Page: 3196
End Page: 3205
Publisher DOI: 10.1002/elps.201600213
PMID: 27649837
Abstract: Complete resolution of hydroxyeicosatetraenoic acid (HETE) enantiomers was achieved using hydroxypropyl-gamma-cyclodextrin (HP-gamma-CD)-modified MEKC. The optimum running conditions were determined to be utilizing a 30 mM phosphate-15 mM borate buffer (pH 9.0) containing 30 mM HP-gamma-CD and 75 mM SDS as the BGE, application of +30 kV as the effective voltage, and carrying out the experiment at 15 degrees C. The eluents were detected at 235 nm. The method was used successfully for the simultaneous separations of (S)- and (R)-enantiomers of regioisomeric 8-, 11-, 12-, and 15-HETEs. Subsequently, the optimized method was applied to evaluate the stereochemistry of 8- and 12-HETEs from the marine red algae, Gracilaria vermiculophylla and Gracilaria arcuata, respectively. The 8-HETE was found to be a mixture of 98% (R)-enantiomer and 2% (S)-enantiomer, while the 12-HETE was a mixture of 98% (S)-enantiomer and 2% (R)-enantiomer. The present study demonstrates that the HP-gamma-CD-modified MEKC method is simple and sensitive and provides unambiguous information on the configuration of natural and synthetic HETEs.
Rights: This is the peer reviewed version of the following article:ELECTROPHORESIS December 2016,37(23-24), p.3196-3205, which has been published in final form at 10.1002/elps.201600213. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
Type: article (author version)
URI: http://hdl.handle.net/2115/67771
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 板橋 豊

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