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Enantioseparation of hydroxyeicosatetraenoic acids by hydroxypropyl-gamma-cyclodextrin-modified micellar electrokinetic chromatography

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タイトル: Enantioseparation of hydroxyeicosatetraenoic acids by hydroxypropyl-gamma-cyclodextrin-modified micellar electrokinetic chromatography
著者: Kodama, Shuji 著作を一覧する
Nakajima, Shota 著作を一覧する
Ozaki, Hiromichi 著作を一覧する
Takemoto, Ryota 著作を一覧する
Itabashi, Yutaka 著作を一覧する
Kuksis, Arnis 著作を一覧する
キーワード: Enantioseparation
Hydroxyeicosatetraenoic acid
Micellar electrokinetic chromatography
発行日: 2016年12月
出版者: Wiley-Blackwell
誌名: Electrophoresis
巻: 37
号: 23-24
開始ページ: 3196
終了ページ: 3205
出版社 DOI: 10.1002/elps.201600213
抄録: Complete resolution of hydroxyeicosatetraenoic acid (HETE) enantiomers was achieved using hydroxypropyl-gamma-cyclodextrin (HP-gamma-CD)-modified MEKC. The optimum running conditions were determined to be utilizing a 30 mM phosphate-15 mM borate buffer (pH 9.0) containing 30 mM HP-gamma-CD and 75 mM SDS as the BGE, application of +30 kV as the effective voltage, and carrying out the experiment at 15 degrees C. The eluents were detected at 235 nm. The method was used successfully for the simultaneous separations of (S)- and (R)-enantiomers of regioisomeric 8-, 11-, 12-, and 15-HETEs. Subsequently, the optimized method was applied to evaluate the stereochemistry of 8- and 12-HETEs from the marine red algae, Gracilaria vermiculophylla and Gracilaria arcuata, respectively. The 8-HETE was found to be a mixture of 98% (R)-enantiomer and 2% (S)-enantiomer, while the 12-HETE was a mixture of 98% (S)-enantiomer and 2% (R)-enantiomer. The present study demonstrates that the HP-gamma-CD-modified MEKC method is simple and sensitive and provides unambiguous information on the configuration of natural and synthetic HETEs.
Rights: This is the peer reviewed version of the following article:ELECTROPHORESIS December 2016,37(23-24), p.3196-3205, which has been published in final form at 10.1002/elps.201600213. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
資料タイプ: article (author version)
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 板橋 豊


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