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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences >
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Enantioseparation of hydroxyeicosatetraenoic acids by hydroxypropyl-gamma-cyclodextrin-modified micellar electrokinetic chromatography
| Title: | Enantioseparation of hydroxyeicosatetraenoic acids by hydroxypropyl-gamma-cyclodextrin-modified micellar electrokinetic chromatography |
| Authors: | Kodama, Shuji Browse this author | | Nakajima, Shota Browse this author | | Ozaki, Hiromichi Browse this author | | Takemoto, Ryota Browse this author | | Itabashi, Yutaka Browse this author →KAKEN DB | | Kuksis, Arnis Browse this author |
| Keywords: | Enantioseparation | | Gracilaria | | Hydroxyeicosatetraenoic acid | | Hydroxypropyl-gamma-cyclodextrin | | Micellar electrokinetic chromatography |
| Issue Date: | Dec-2016 |
| Publisher: | Wiley-Blackwell |
| Journal Title: | Electrophoresis |
| Volume: | 37 |
| Issue: | 23-24 |
| Start Page: | 3196 |
| End Page: | 3205 |
| Publisher DOI: | 10.1002/elps.201600213 |
| PMID: | 27649837 |
| Abstract: | Complete resolution of hydroxyeicosatetraenoic acid (HETE) enantiomers was achieved using hydroxypropyl-gamma-cyclodextrin (HP-gamma-CD)-modified MEKC. The optimum running conditions were determined to be utilizing a 30 mM phosphate-15 mM borate buffer (pH 9.0) containing 30 mM HP-gamma-CD and 75 mM SDS as the BGE, application of +30 kV as the effective voltage, and carrying out the experiment at 15 degrees C. The eluents were detected at 235 nm. The method was used successfully for the simultaneous separations of (S)- and (R)-enantiomers of regioisomeric 8-, 11-, 12-, and 15-HETEs. Subsequently, the optimized method was applied to evaluate the stereochemistry of 8- and 12-HETEs from the marine red algae, Gracilaria vermiculophylla and Gracilaria arcuata, respectively. The 8-HETE was found to be a mixture of 98% (R)-enantiomer and 2% (S)-enantiomer, while the 12-HETE was a mixture of 98% (S)-enantiomer and 2% (R)-enantiomer. The present study demonstrates that the HP-gamma-CD-modified MEKC method is simple and sensitive and provides unambiguous information on the configuration of natural and synthetic HETEs. |
| Rights: | This is the peer reviewed version of the following article:ELECTROPHORESIS December 2016,37(23-24), p.3196-3205, which has been published in final form at 10.1002/elps.201600213. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/67771 |
| Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 板橋 豊
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