| Title: | Putative RNA viral sequences detected in an Ixodes scapularis-derived cell line |
| Authors: | Nakao, Ryo Browse this author →KAKEN DB |
| Matsuno, Keita Browse this author →KAKEN DB |
| Qiu, Yongjin Browse this author |
| Marilyama, Junki Browse this author |
| Eguchi, Nao Browse this author |
| Nao, Naganori Browse this author |
| Kajihara, Masahiro Browse this author →KAKEN DB |
| Yoshii, Kentaro Browse this author →KAKEN DB |
| Sawa, Hirofumi Browse this author →KAKEN DB |
| Takada, Ayato Browse this author →KAKEN DB |
| Sugimoto, Chihiro Browse this author →KAKEN DB |
| Keywords: | Bunyavirus |
| Iflavirus |
| ISE6 |
| Ixodes scapularis |
| Ixodes scapularis-associated virus-1 |
| Issue Date: | 22-Jan-2018 |
| Publisher: | Elsevier |
| Journal Title: | Ticks and Tick-Borne Diseases |
| Volume: | 8 |
| Issue: | 1 |
| Start Page: | 103 |
| End Page: | 111 |
| Publisher DOI: | 10.1016/j.ttbdis.2016.10.005 |
| PMID: | 27769656 |
| Abstract: | Ticks harbour various microorganisms, some of which act as pathogens of humans and animals. The recent advancement of genome sequencing technologies revealed that a wide range of previously unrecognised microorganisms exist in ticks. Continuous cell lines established from ticks could play a key role in the isolation of such microorganisms; however, tick cells themselves have been known to harbour symbiotic microorganisms. The present study aimed to characterise putative RNA viral sequences detected in the culture supernatant of one of the most frequently used tick cell lines, ISE6, which was derived from embryos of the blacklegged tick Ixodes scapularis. Viral particles purified from the culture supernatant were used for RNA extraction, followed by Illumina sequencing. The reads were de novo assembled and the resulting contigs were annotated by tBLASTx search. The results suggested that there were at least five putative viral sequences of four phylogenetically distinct lineages in ISE6 cells. The predominant viral sequence found in ISE6 cells, designated L scapularis iflavirus, was a member of the family Iflaviridae, which is an arthropod-infecting virus group. We also identified L and M segments of the family Bunyaviridae, which could not be classified into any of the five known genera, and a potential capsid protein related to Drosophila A virus. In addition to these previously unrecognised viruses, ISE6 was revealed to harbour a putative genome sequence of L scapularis-associated virus-1, which was reported in a recent metagenomic study of L scapularis itself. All the five putative viral sequences were detected by RT-PCR in both ISE6 cells and the culture supernatant. Electron microscopic analysis showed the existence of spherical virions with a varying diameter of 50-70 nm in the culture supernatant of ISE6 cells. Further studies are required to investigate the potential roles of ISE6-associated viruses in ticks. (C) 2017 Elsevier GmbH. All rights reserved. |
| Rights: | ©2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/68171 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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