| Title: | Inducible Transposition of a Heat-Activated Retrotransposon in Tissue Culture |
| Authors: | Masuta, Yukari Browse this author |
| Nozawa, Kosuke Browse this author |
| Takagi, Hiroki Browse this author |
| Yaegashi, Hiroki Browse this author |
| Tanaka, Keisuke Browse this author |
| Ito, Tasuku Browse this author |
| Saito, Hideyuki Browse this author →KAKEN DB |
| Kobayashi, Hisato Browse this author |
| Matsunaga, Wataru Browse this author |
| Masuda, Seiji Browse this author |
| Kato, Atsushi Browse this author →KAKEN DB |
| Ito, Hidetaka Browse this author →KAKEN DB |
| Keywords: | Brassicaceae |
| Heat stress |
| ONSEN |
| Tissue culture |
| Transposon |
| Issue Date: | 1-Feb-2017 |
| Publisher: | Oxford University Press |
| Journal Title: | Plant and Cell Physiology |
| Volume: | 58 |
| Issue: | 2 |
| Start Page: | 375 |
| End Page: | 384 |
| Publisher DOI: | 10.1093/pcp/pcw202 |
| PMID: | 28013279 |
| Abstract: | A transposition of a heat-activated retrotransposon named ONSEN required compromise of a small RNA-mediated epigenetic regulation that includes RNA-directed DNA methylation (RdDM) machinery after heat treatment. In the current study, we analyzed the transcriptional and transpositional activation of ONSEN to better understand the underlying molecular mechanism involved in the maintenance and/or induction of transposon activation in plant tissue culture. We found the transposition of heat-primed ONSEN during tissue culture independently of RdDM mutation. The heat activation of ONSEN transcripts was not significantly up-regulated in tissue culture compared with that in heat-stressed seedlings, indicating that the transposition of ONSEN was regulated independently of the transcript level. RdDM-related genes were up-regulated by heat stress in both tissue culture and seedlings. The level of DNA methylation of ONSEN did not show any change in tissue culture, and the amount of ONSEN-derived small RNAs was not affected by heat stress. The results indicated that the transposition of ONSEN was regulated by an alternative mechanism in addition to the RdDM-mediated epigenetic regulation in tissue culture. We applied the tissue culture-induced transposition of ONSEN to Japanese radish, an important breeding species of the family Brassicaceae. Several new insertions were detected in a regenerated plant derived from heat-stressed tissues and its self-fertilized progeny, revealing the possibility of molecular breeding without genetic modification. |
| Rights: | This is a pre-copyedited, author-produced PDF of an article accepted for publication in Plant & Cell Physiology following peer review. The version of record Plant Cell Physiol (2017) 58 (2): 375-384 is available online at: https://academic.oup.com/pcp/article-lookup/doi/10.1093/pcp/pcw202 |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/68271 |
| Appears in Collections: | 理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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