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Inducible Transposition of a Heat-Activated Retrotransposon in Tissue Culture

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Title: Inducible Transposition of a Heat-Activated Retrotransposon in Tissue Culture
Authors: Masuta, Yukari Browse this author
Nozawa, Kosuke Browse this author
Takagi, Hiroki Browse this author
Yaegashi, Hiroki Browse this author
Tanaka, Keisuke Browse this author
Ito, Tasuku Browse this author
Saito, Hideyuki Browse this author →KAKEN DB
Kobayashi, Hisato Browse this author
Matsunaga, Wataru Browse this author
Masuda, Seiji Browse this author
Kato, Atsushi Browse this author →KAKEN DB
Ito, Hidetaka Browse this author →KAKEN DB
Keywords: Brassicaceae
Heat stress
Tissue culture
Issue Date: 1-Feb-2017
Publisher: Oxford University Press
Journal Title: Plant and Cell Physiology
Volume: 58
Issue: 2
Start Page: 375
End Page: 384
Publisher DOI: 10.1093/pcp/pcw202
PMID: 28013279
Abstract: A transposition of a heat-activated retrotransposon named ONSEN required compromise of a small RNA-mediated epigenetic regulation that includes RNA-directed DNA methylation (RdDM) machinery after heat treatment. In the current study, we analyzed the transcriptional and transpositional activation of ONSEN to better understand the underlying molecular mechanism involved in the maintenance and/or induction of transposon activation in plant tissue culture. We found the transposition of heat-primed ONSEN during tissue culture independently of RdDM mutation. The heat activation of ONSEN transcripts was not significantly up-regulated in tissue culture compared with that in heat-stressed seedlings, indicating that the transposition of ONSEN was regulated independently of the transcript level. RdDM-related genes were up-regulated by heat stress in both tissue culture and seedlings. The level of DNA methylation of ONSEN did not show any change in tissue culture, and the amount of ONSEN-derived small RNAs was not affected by heat stress. The results indicated that the transposition of ONSEN was regulated by an alternative mechanism in addition to the RdDM-mediated epigenetic regulation in tissue culture. We applied the tissue culture-induced transposition of ONSEN to Japanese radish, an important breeding species of the family Brassicaceae. Several new insertions were detected in a regenerated plant derived from heat-stressed tissues and its self-fertilized progeny, revealing the possibility of molecular breeding without genetic modification.
Rights: This is a pre-copyedited, author-produced PDF of an article accepted for publication in Plant & Cell Physiology following peer review. The version of record Plant Cell Physiol (2017) 58 (2): 375-384 is available online at:
Type: article (author version)
Appears in Collections:理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 伊藤 秀臣

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