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Scalable preparation of poly(ethyleneglycol)-grafted siRNA-loaded lipid nanoparticles using a commercially available fluidic device and tangential flow filtration

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タイトル: Scalable preparation of poly(ethyleneglycol)-grafted siRNA-loaded lipid nanoparticles using a commercially available fluidic device and tangential flow filtration
著者: Sakurai, Yu 著作を一覧する
Hada, Tomoya 著作を一覧する
Harashima, Hideyoshi 著作を一覧する
キーワード: Liposomes
nanoparticles
DNA/oligonucleotide delivery
siRNA
hepatocytes
liver
large-scale preparation
発行日: 2017年 2月10日
出版者: Taylor & Francis
誌名: Journal of biomaterials science. Polymer edition
巻: 28
号: 10-12
開始ページ: 1086
終了ページ: 1096
出版社 DOI: 10.1080/09205063.2017.1291297
抄録: While a number of siRNA delivery systems have been developed, the methods used in their preparation are not suitable for large-scale production. We herein report on methodology for the large-scale preparation of liposomal siRNA using a fluidic device and tangential flow filtration (TFF). A number of studies have appeared on the use of fluidic devices for preparing and purifying liposomes, but no systematic information regarding appropriate membrane type of commercially available apparatus is available. The findings reported herein indicate that, under optimized conditions, a microfluidic device and TFF can be used to produce siRNA lipid nanoparticles with the same characteristics as traditional ones'. The in vivo silencing efficiency of these lipid nanoparticles in the liver was comparable to laboratory-produced nanoparticles. In addition, con-focal laser scanning microscopy analyses revealed that they accumulated in the liver accumulation at the same levels as particles produced by batch-type and continuous-type procedures. This methodology has the potential to contribute to the advancement of this process from basic research to clinical studies of liposomal DDS.
Rights: This is an Accepted Manuscript of an article published by Taylor & Francis in J. Biomater. Sci.-Polym. Ed. on 2017-02-10, available online: http://www.tandfonline.com/10.1080/09205063.2017.1291297
資料タイプ: article (author version)
URI: http://hdl.handle.net/2115/68304
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 櫻井 遊

 

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