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Scalable preparation of poly(ethyleneglycol)-grafted siRNA-loaded lipid nanoparticles using a commercially available fluidic device and tangential flow filtration

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Title: Scalable preparation of poly(ethyleneglycol)-grafted siRNA-loaded lipid nanoparticles using a commercially available fluidic device and tangential flow filtration
Authors: Sakurai, Yu Browse this author →KAKEN DB
Hada, Tomoya Browse this author
Harashima, Hideyoshi Browse this author →KAKEN DB
Keywords: Liposomes
nanoparticles
DNA/oligonucleotide delivery
siRNA
hepatocytes
liver
large-scale preparation
Issue Date: 10-Feb-2017
Publisher: Taylor & Francis
Journal Title: Journal of biomaterials science. Polymer edition
Volume: 28
Issue: 10-12
Start Page: 1086
End Page: 1096
Publisher DOI: 10.1080/09205063.2017.1291297
PMID: 28157422
Abstract: While a number of siRNA delivery systems have been developed, the methods used in their preparation are not suitable for large-scale production. We herein report on methodology for the large-scale preparation of liposomal siRNA using a fluidic device and tangential flow filtration (TFF). A number of studies have appeared on the use of fluidic devices for preparing and purifying liposomes, but no systematic information regarding appropriate membrane type of commercially available apparatus is available. The findings reported herein indicate that, under optimized conditions, a microfluidic device and TFF can be used to produce siRNA lipid nanoparticles with the same characteristics as traditional ones'. The in vivo silencing efficiency of these lipid nanoparticles in the liver was comparable to laboratory-produced nanoparticles. In addition, con-focal laser scanning microscopy analyses revealed that they accumulated in the liver accumulation at the same levels as particles produced by batch-type and continuous-type procedures. This methodology has the potential to contribute to the advancement of this process from basic research to clinical studies of liposomal DDS.
Rights: This is an Accepted Manuscript of an article published by Taylor & Francis in J. Biomater. Sci.-Polym. Ed. on 2017-02-10, available online: http://www.tandfonline.com/10.1080/09205063.2017.1291297
Type: article (author version)
URI: http://hdl.handle.net/2115/68304
Appears in Collections:薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 櫻井 遊

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