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Novel endogenous simian retroviral integrations in Vero cells: implications for quality control of a human vaccine cell substrate

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Title: Novel endogenous simian retroviral integrations in Vero cells: implications for quality control of a human vaccine cell substrate
Authors: Sakuma, Chisato Browse this author
Sekizuka, Tsuyoshi Browse this author
Kuroda, Makoto Browse this author
Kasai, Fumio Browse this author
Saito, Kyoko Browse this author
Ikeda, Masaki Browse this author
Yamaji, Toshiyuki Browse this author
Osada, Naoki Browse this author →KAKEN DB
Hanada, Kentaro Browse this author
Issue Date: 12-Jan-2018
Publisher: Nature Publishing Group
Journal Title: Scientific reports
Volume: 8
Start Page: 644
Publisher DOI: 10.1038/s41598-017-18934-2
Abstract: African green monkey (AGM)-derived Vero cells have been utilized to produce various human vaccines. The Vero cell genome harbors a variety of simian endogenous type D retrovirus (SERV) sequences. In this study, a transcriptome analysis showed that DNA hypomethylation released the epigenetic repression of SERVs in Vero cells. Moreover, comparative genomic analysis of three Vero cell sublines and an AGM reference revealed that the genomes of the sublines have similar to 80 SERV integrations. Among them, similar to 60 integrations are present within all three cell sublines and absent from the reference sequence. At least several of these integrations consist of complete SERV proviruses. These results strongly suggest that SERVs integrated in the genome of Vero cells did not retrotranspose after the establishment of the cell lineage as far as cells were maintained under standard culture and passage conditions, providing a scientific basis for controlling the quality of pharmaceutical cell substrates and their derived biologics.
Rights: https://creativecommons.org/licenses/by/4.0/
Type: article
URI: http://hdl.handle.net/2115/68378
Appears in Collections:情報科学院・情報科学研究院 (Graduate School of Information Science and Technology / Faculty of Information Science and Technology) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 長田 直樹

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