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Activation of nucleotide-binding domain-like receptor containing protein 3 inflammasome in dendritic cells and macrophages by Streptococcus sanguinis

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/68426

Title: Activation of nucleotide-binding domain-like receptor containing protein 3 inflammasome in dendritic cells and macrophages by Streptococcus sanguinis
Authors: Saeki, Ayumi Browse this author →KAKEN DB
Suzuki, Toshihiko Browse this author
Hasebe, Akira Browse this author →KAKEN DB
Kamezaki, Ryousuke Browse this author
Fujita, Mari Browse this author
Nakazawa, Futoshi Browse this author
Shibata, Ken-Ichiro Browse this author →KAKEN DB
Issue Date: Mar-2017
Publisher: Wiley-Blackwell
Journal Title: Cellular Microbiology
Volume: 19
Issue: 3
Start Page: e12663
Publisher DOI: 10.1111/cmi.12663
PMID: 27601185
Abstract: Streptococcus sanguinis is frequently isolated from the blood of patients with infective endocarditis and contributes to the pathology of this disease through induction of interleukin (IL)-1β responsible for the development of the disease. However, the mechanism of IL-1β induction remains unknown. In this study, S. sanguinis activated a murine dendritic cell (DC) to induce IL-1β and this activity was attenuated by silencing the mRNAs of nucleotide-binding domain-like receptor containing protein 3 (NLRP3) and caspase-1. S. sanguinis induced IL-1β production in murine bone marrow-derived macrophage (BMM), but this activity was significantly reduced in BMMs from NLRP3-, apoptosis-associated speck-like protein containing a caspase-recruitment domain-, and caspase-1-deficient mice. DC phagocytosed S. sanguinis cells, followed by the release of adenosine triphosphate (ATP). The ATP-degradating enzyme attenuated the release of ATP and IL-1β. The inhibitors for ATP receptor reduced IL-1β release in DC. These results strongly suggest that S. sanguinis has the activity to induce IL-1β through the NLRP3 inflammasome in macrophage and DC and interaction of purinergic receptors with ATP released is involved in expression of the activity.
Rights: This is the peer reviewed version of the following article: [Activation of nucleotide-binding domain-like receptor containing protein 3 inflammasome in dendritic cells and macrophages by Streptococcus sanguinis.], which has been published in final form at [http://doi.org/10.1111/cmi.12663]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
Type: article (author version)
URI: http://hdl.handle.net/2115/68426
Appears in Collections:歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 柴田 健一郎

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