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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Science / Faculty of Science >
Peer-reviewed Journal Articles, etc >
Heme Iron Coordination Structure of Heme Transport Protein HutB from Vibrio Cholerae
| Title: | Heme Iron Coordination Structure of Heme Transport Protein HutB from Vibrio Cholerae |
| Authors: | Uchida, Takeshi Browse this author →KAKEN DB | | Funamizu, Takumi Browse this author | | Ogura, Mariko Browse this author | | Ishimori, Koichiro Browse this author →KAKEN DB |
| Issue Date: | Aug-2017 |
| Publisher: | Chemical Society of Japan |
| Journal Title: | Bulletin of the Chemical Society of Japan |
| Volume: | 90 |
| Issue: | 8 |
| Start Page: | 924 |
| End Page: | 930 |
| Publisher DOI: | 10.1246/bcsj.20170104 |
| Abstract: | HutB is a putative heme transport protein located in the periplasmic space in Vibrio cholerae. Here, we purified HutB and characterized its heme binding properties. An analysis of the Soret band showed that there are two types of heme binding geometries depending on the heme concentration: 404-nm species are dominant at lower concentrations of heme, and 394-nm species dominate at higher concentrations. Moreover, a mutational study revealed that either Tyr65 or Tyr198 binds heme with the help of histidine, a property shared with another V. cholerae heme transport protein, HutX, despite the absence of sequence similarity, indicating that HutB acts as a heme transport protein in the periplasm. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/68744 |
| Appears in Collections: | 理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 内田 毅
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