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Involvement of the nuclear progestin receptor in LH-induced expression of membrane type 2-matrix metalloproteinase required for follicle rupture during ovulation in the medaka, Oryzias latipes
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| Title: | Involvement of the nuclear progestin receptor in LH-induced expression of membrane type 2-matrix metalloproteinase required for follicle rupture during ovulation in the medaka, Oryzias latipes |
| Authors: | Ogiwara, Katsueki Browse this author →KAKEN DB | | Takahashi, Takayuki Browse this author →KAKEN DB |
| Keywords: | Medaka | | Ovulation | | LH | | Mmp15 | | Nuclear progestin receptor |
| Issue Date: | 15-Jul-2017 |
| Publisher: | Elsevier |
| Journal Title: | Molecular and cellular endocrinology |
| Volume: | 450 |
| Start Page: | 54 |
| End Page: | 63 |
| Publisher DOI: | 10.1016/j.mce.2017.04.016 |
| PMID: | 28416325 |
| Abstract: | Hormonal regulation of the expression of Mmp15, a proteolytic enzyme indispensable for ovulation in the teleost medaka, was investigated. In an in vitro culture system using preovulatory follicles, Mmp15 expression and ovulation were induced in the presence of recombinant luteinizing hormone (rLh). Both rLh-induced Mmp15 expression and ovulation were 17 alpha, 20 beta-dihydroxy-4-pregnen-3-one-dependent, suggesting the involvement of a nuclear progestin receptor (Pgr). In vitro follicle ovulation and Mmp15 expression were reduced by treatment with the Pgr antagonist RU-486. Like Pgr, the transcription factor CCAAT/enhancer-binding protein beta (Cebpb) was induced by rLh. ChIP analyses indicated that Pgr and Cebpb bound to the mmpl5 promoter region. These results indicate that the rLh-induced expression of Mmp15 is mediated by Pgr and Cebpb. A differential timing of expression of Pgr and Cebpb in the preovulatory follicles appears to explain the considerably long time-lag from the pgr gene activation to mmpl5 gene expression. (C) 2017 Elsevier B.V. All rights reserved. |
| Rights: | © 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | | http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/68833 |
| Appears in Collections: | 理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 荻原 克益
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