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Bacterial histo-blood group antigens contributing to genotype-dependent removal of human noroviruses with a microfiltration membrane

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Title: Bacterial histo-blood group antigens contributing to genotype-dependent removal of human noroviruses with a microfiltration membrane
Authors: Amarasiri, Mohan Browse this author
Hashiba, Satoshi Browse this author
Miura, Takayuki Browse this author
Nakagomi, Toyoko Browse this author →KAKEN DB
Nakagomi, Osamu Browse this author →KAKEN DB
Ishii, Satoshi Browse this author
Okabe, Satoshi Browse this author →KAKEN DB
Sano, Daisuke Browse this author →KAKEN DB
Keywords: Extracellular polymeric substance
Histo-blood group antigen
Human norovirus
Lipopolysaccharide
Microfiltration
Membrane bioreactor
Issue Date: 15-May-2016
Publisher: Elsevier
Journal Title: Water research
Volume: 95
Start Page: 383
End Page: 391
Publisher DOI: 10.1016/j.watres.2016.04.018
PMID: 27095709
Abstract: We demonstrated the genotype-dependent removal of human norovirus particles with a microfiltration (MF) membrane in the presence of bacteria bearing histo-blood group antigens (HBGAs). Three genotypes (GII.3, GII.4, and GII.6) of norovirus-like particles (NoVLPs) were mixed with three bacterial strains (Enterobacter sp. SENG-6, Escherichia coli 086:K61:B7, and Staphylococcus epidermidis), respectively, and the mixture was filtered with an MF membrane having a nominal pore size of 0.45 mu m. All NoVLP genotypes were rejected by the MF membrane in the presence of Enterobacter sp. SENG-6, which excreted HBGAs as extracellular polymeric substances (EPS). This MF membrane removal of NoVLPs was not significant when EPS was removed from cells of Enterobacter sp. SENG-6. GII.6 NoVLP was not rejected with the MF membrane in the presence of E. coli 086:K61:B7, but the removal of EPS of E. coli 086:K61:B7 increased the removal efficiency due to the interaction of NoVLPs with the exposed B-antigen in lipopolysaccharide (LPS) of E. coli 086:K61:B7. No MF membrane removal of all three genotypes was observed when S. epidermidis, an HBGA-negative strain, was mixed with NoVLPs. These results demonstrate that the location of HBGAs on bacterial cells is an important factor in determining the genotype-dependent removal efficiency of norovirus particles with the MF membrane. The presence of HBGAs in mixed liquor suspended solids from a membrane bioreactor (MBR) pilot plant was confirmed by immune-transmission electron microscopy, which implies that bacterial HBGAs can contribute to the genotype-dependent removal of human noroviruses with MBR using MF membrane.
Rights: © 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
http://creativecommons.org/licenses/by-nc-nd/4.0/
Type: article (author version)
URI: http://hdl.handle.net/2115/70214
Appears in Collections:工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 佐野 大輔

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