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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Information Science and Technology / Faculty of Information Science and Technology >
Peer-reviewed Journal Articles, etc >
Feasibility of in vivo three-dimensional T-2(*) mapping using dicarboxy-PROXYL and CW-EPR-based single-point imaging
| Title: | Feasibility of in vivo three-dimensional T-2(*) mapping using dicarboxy-PROXYL and CW-EPR-based single-point imaging |
| Authors: | Kubota, Harue Browse this author | | Komarov, Denis A. Browse this author | | Yasui, Hironobu Browse this author →KAKEN DB | | Matsumoto, Shingo Browse this author | | Inanami, Osamu Browse this author →KAKEN DB | | Kirilyuk, Igor A. Browse this author | | Khramtsov, Valery V. Browse this author | | Hirata, Hiroshi Browse this author →KAKEN DB |
| Keywords: | Nitroxyl radical | | Clonogenic assay | | In vivo nitroxyl radical kinetics | | In vivo EPR | | T-2* mapping | | Single-point imaging |
| Issue Date: | Jun-2017 |
| Publisher: | Springer |
| Journal Title: | Magnetic Resonance Materials in Physics, Biology and Medicine |
| Volume: | 30 |
| Issue: | 3 |
| Start Page: | 291 |
| End Page: | 298 |
| Publisher DOI: | 10.1007/s10334-016-0606-8 |
| Abstract: | Objectives The aim of this study was to demonstrate the feasibility of in vivo three-dimensional (3D) relaxation time T-2* mapping of a dicarboxy-PROXYL radical using continuous-wave electron paramagnetic resonance (CW-EPR) imaging. Materials and methods Isotopically substituted dicarboxy-PROXYL radicals, 3,4-dicarboxy-2,2,5,5-tetra(H-2(3)) methylpyrrolidin-( 3,4-H-2(2))-(1-N-15)-1-oxyl (H-2, N-15-DCP) and 3,4-dicarboxy-2,2,5,5-tetra(H-2(3)) methylpyrrolidin-(3,4-H-2(2))1- oxyl (H-2-DCP), were used in the study. A clonogenic cell survival assay was performed with the H-2-DCP radical using squamous cell carcinoma (SCC VII) cells. The time course of EPR signal intensities of intravenously injected H-2, N-15-DCP and H-2-DCP radicals were determined in tumor-bearing hind legs of mice (C3H/HeJ, male, n = 5). CW-EPR-based single-point imaging (SPI) was performed for 3D T-2* mapping. Results H-2-DCP radical did not exhibit cytotoxicity at concentrations below 10 mM. The in vivo half-life of H-2, N-15-DCP in tumor tissues was 24.7 +/- 2.9 min (mean +/- standard deviation [SD], n = 5). The in vivo time course of the EPR signal intensity of the H-2, N-15-DCP radical showed a plateau of 10.2 +/- 1.2 min (mean +/- SD) where the EPR signal intensity remained at more than 90% of the maximum intensity. During the plateau, in vivo 3D T-2* maps with H-2, N-15-DCP were obtained from tumor-bearing hind legs, with a total acquisition time of 7.5 min. Conclusion EPR signals of H-2, N-15-DCP persisted long enough after bolus intravenous injection to conduct in vivo 3D T-2* mapping with CW-EPR-based SPI. |
| Rights: | "The original publication is available at www.springerlink.com". |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/70631 |
| Appears in Collections: | 情報科学院・情報科学研究院 (Graduate School of Information Science and Technology / Faculty of Information Science and Technology) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 平田 拓
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