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Characterization of a heat-activated retrotransposon in Vigna angularis

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/70664

Title: Characterization of a heat-activated retrotransposon in Vigna angularis
Authors: Masuta, Yukari Browse this author
Kawabe, Akira Browse this author
Nozawa, Kosuke Browse this author
Naito, Ken Browse this author
Kato, Atsushi Browse this author →KAKEN DB
Ito, Hidetaka Browse this author →KAKEN DB
Keywords: heat stress
adzuki
Vigna angularis
ONSEN
retrotransposon
Issue Date: Mar-2018
Publisher: Japanese Society of Breeding
Journal Title: Breeding Science
Volume: 68
Issue: 2
Start Page: 168
End Page: 176
Publisher DOI: 10.1270/jsbbs.17085
Abstract: In plants, several transposable elements are conserved across species. We found a homolog of ONSEN, which is a heat-activated retrotransposon originally isolated from Arabidopsis thaliana, in Vigna. The ONSEN-like elements (VaONS) were detected in all the analyzed Japanese accessions of Vigna angularis (adzuki bean) by Southern blot analysis. However, VaONS sequences were observed to be polymorphic in the different accessions. Interestingly, extrachromosomal DNA (ecDNA) was detected in some accessions of adzuki bean, indicating the conserved heat-activation of VaONS. Furthermore, we successfully induced retrotransposition of VaONS in adzuki plant regenerated through callus. Findings of our study should provide a new tool for molecular breeding of adzuki bean.
Type: article
URI: http://hdl.handle.net/2115/70664
Appears in Collections:理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 伊藤 秀臣

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