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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Science / Faculty of Science >
Peer-reviewed Journal Articles, etc >
Characterization of a heat-activated retrotransposon in Vigna angularis
| Title: | Characterization of a heat-activated retrotransposon in Vigna angularis |
| Authors: | Masuta, Yukari Browse this author | | Kawabe, Akira Browse this author | | Nozawa, Kosuke Browse this author | | Naito, Ken Browse this author | | Kato, Atsushi Browse this author →KAKEN DB | | Ito, Hidetaka Browse this author →KAKEN DB |
| Keywords: | heat stress | | adzuki | | Vigna angularis | | ONSEN | | retrotransposon |
| Issue Date: | Mar-2018 |
| Publisher: | Japanese Society of Breeding |
| Journal Title: | Breeding Science |
| Volume: | 68 |
| Issue: | 2 |
| Start Page: | 168 |
| End Page: | 176 |
| Publisher DOI: | 10.1270/jsbbs.17085 |
| Abstract: | In plants, several transposable elements are conserved across species. We found a homolog of ONSEN, which is a heat-activated retrotransposon originally isolated from Arabidopsis thaliana, in Vigna. The ONSEN-like elements (VaONS) were detected in all the analyzed Japanese accessions of Vigna angularis (adzuki bean) by Southern blot analysis. However, VaONS sequences were observed to be polymorphic in the different accessions. Interestingly, extrachromosomal DNA (ecDNA) was detected in some accessions of adzuki bean, indicating the conserved heat-activation of VaONS. Furthermore, we successfully induced retrotransposition of VaONS in adzuki plant regenerated through callus. Findings of our study should provide a new tool for molecular breeding of adzuki bean. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/70664 |
| Appears in Collections: | 理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 伊藤 秀臣
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