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High-Sensitivity and High-Resolution In Situ Hybridization of Coding and Long Non-coding RNAs in Vertebrate Ovaries and Testes
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| Title: | High-Sensitivity and High-Resolution In Situ Hybridization of Coding and Long Non-coding RNAs in Vertebrate Ovaries and Testes |
| Authors: | Takei, Natsumi Browse this author | | Nakamura, Takuma Browse this author | | Kawamura, Shohei Browse this author | | Takada, Yuki Browse this author | | Satoh, Yui Browse this author | | Kimura, Atsushi P. Browse this author →KAKEN DB | | Kotani, Tomoya Browse this author →KAKEN DB |
| Keywords: | RNA localization | | Post-transcriptional regulation | | Germ cell | | Tissue | | Organ |
| Issue Date: | 1-Mar-2018 |
| Publisher: | BioMed Central |
| Journal Title: | Biological procedures online |
| Volume: | 20 |
| Start Page: | 6 |
| Publisher DOI: | 10.1186/s12575-018-0071-z |
| Abstract: | Background: Subcellular localization of coding and non-coding RNAs has emerged as major regulatory mechanisms of gene expression in various cell types and many organisms. However, techniques that enable detection of the subcellular distribution of these RNAs with high sensitivity and high resolution remain limited, particularly in vertebrate adult tissues and organs. In this study, we examined the expression and localization of mRNAs encoding Pou5f1/Oct4, Mos, Cyclin B1 and Deleted in Azoospermia-like (Dazl) in zebrafish and mouse ovaries by combining tyramide signal amplification (TSA)-based in situ hybridization with paraffin sections which can preserve cell morphology of tissues and organs at subcellular levels. In addition, the distribution of a long non-coding RNA (lncRNA), lncRNA-HSVIII, in mouse testes was examined by the same method. Results: The mRNAs encoding Mos, Cyclin B1 and Dazl were found to assemble into distinct granules that were distributed in different subcellular regions of zebrafish and mouse oocytes, suggesting conserved and specific regulations of these mRNAs. The lncRNA-HSVIII was first detected in the nucleus of spermatocytes at prophase I of the meiotic cell cycle and was then found in the cytoplasm of round spermatids, revealing expression patterns of lncRNA during germ cell development. Collectively, the in situ hybridization method demonstrated in this study achieved the detection and comparison of precise distribution patterns of coding and non-coding RNAs at subcellular levels in single cells of adult tissues and organs. Conclusions: This high-sensitivity and high-resolution in situ hybridization is applicable to many vertebrate species and to various tissues and organs and will be useful for studies on the subcellular regulation of gene expression at the level of RNA localization. |
| Rights: | http://creativecommons.org/licenses/by/4.0 |
| Type: | article |
| URI: | http://hdl.handle.net/2115/70666 |
| Appears in Collections: | 理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 小谷 友也
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