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Real-time imaging of actin filaments in the zebrafish oocyte and embryo

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Title: Real-time imaging of actin filaments in the zebrafish oocyte and embryo
Authors: Nukada, Yumiko Browse this author
Horie, Mayu Browse this author
Fukui, Akimasa Browse this author
Kotani, Tomoya Browse this author →KAKEN DB
Yamashita, Masakane Browse this author →KAKEN DB
Keywords: vertebrate
oocyte maturation
transgenic fish
live imaging
Issue Date: Sep-2015
Journal Title: Cytoskeleton
Volume: 72
Issue: 9
Start Page: 491
End Page: 501
Publisher DOI: 10.1002/cm.21253
PMID: 26335601
Abstract: Dynamic changes of cytoplasmic and cortical actin filaments drive various cellular and developmental processes. Although real-time imaging of actin filaments in living cells has been developed, imaging of actin filaments in specific cells of living organisms remains limited, particularly for the analysis of gamete formation and early embryonic development. Here, we report the production of transgenic zebrafish expressing the C-terminus of Moesin, an actin filament-binding protein, fused with green fluorescent protein or red fluorescent protein (GFP/RFP-MoeC), under the control of a cyclin B1 promoter. GFP/RFP-MoeC was expressed maternally, which labels the cortical actin cytoskeleton of blastula-stage cells. High levels of GFP/RFP fluorescence were detected in the adult ovary and testis. In the ovaries, GFP/RFP-MoeC was expressed in oocytes but not in follicle cells, which allows us to clearly visualize the organization of actin filaments in different stages of the oocyte. Using full-grown oocytes, we revealed the dynamic changes of actin columns assembled in the cortical cytoplasm during oocyte maturation. The number of columns slightly decreased in the early period before germinal vesicle breakdown (GVBD) and then significantly decreased at GVBD, followed by recovery after GVBD. Our transgenic fish are useful for analyzing the dynamics of actin filaments in oogenesis and early embryogenesis.
Rights: This is the peer reviewed version of the following article: cytoskeleton 72(9) September 2015, pp.491-501 which has been published in final form at This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
Type: article (author version)
Appears in Collections:理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 小谷 友也

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