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STAP-2 interacts with Pyk2 and enhances Pyk2 activity in T-cells
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| Title: | STAP-2 interacts with Pyk2 and enhances Pyk2 activity in T-cells |
| Authors: | Saitoh, Kodai Browse this author | | Tsuchiya, Takuya Browse this author | | Kashiwakura, Jun-ichi Browse this author | | Muromoto, Ryuta Browse this author →KAKEN DB | | Kitai, Yuichi Browse this author →KAKEN DB | | Sekine, Yuichi Browse this author | | Oritani, Kenji Browse this author | | Matsuda, Tadashi Browse this author →KAKEN DB |
| Keywords: | Pyk2 | | STAP-2 | | SDF-l alpha | | T-cell | | Chemotaxis |
| Issue Date: | 17-Jun-2017 |
| Publisher: | Elsevier |
| Journal Title: | Biochemical and biophysical research communications |
| Volume: | 488 |
| Issue: | 1 |
| Start Page: | 81 |
| End Page: | 87 |
| Publisher DOI: | 10.1016/j.bbrc.2017.05.010 |
| PMID: | 28478037 |
| Abstract: | STAP-2 is an adaptor molecule regulating several signaling pathways, including TLRs and cytokine/chemokine receptors in immune cells. We previously reported that STAP-2 enhances SDF-1 a-induced Vavl/Racl-mediated T-cell chemotaxis. However, the detailed mechanisms of STAP-2 involvement in enhancing T-cell chemotaxis remain unknown. In the present study, we demonstrate that STAP-2 directly interacts with Pyk2, which is a key molecule in the regulation of SDF-la/CXCR4-mediated T-cell chemotaxis, and increases phosphorylation of Pyk2. Pyk2 itself can induce STAP-2 Y250 phosphorylation, and this phosphorylation is critical for maximal interactions between STAP-2 and Pyk2. Finally, SDF-1 a induced T-cell chemotaxis is inhibited by treatment with Pyk2 siRNA or AG17, an inhibitor of Pyk2, in Jurkat cells overexpressing STAP-2. Taken together, the Pyk2/STAP-2 interaction is a novel mechanism to regulate SDF-1 alpha-dependent T-cell chemotaxis. |
| Rights: | ©2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | | http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/70743 |
| Appears in Collections: | 薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 松田 正
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