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Reaction intermediates in the heme degradation reaction by HutZ from Vibrio cholerae
| Title: | Reaction intermediates in the heme degradation reaction by HutZ from Vibrio cholerae |
| Authors: | Uchida, Takeshi Browse this author →KAKEN DB | | Sekine, Yukari Browse this author | | Dojun, Nobuhiko Browse this author | | Lewis-Ballester, Ariel Browse this author | | Ishigami, Izumi Browse this author | | Matsui, Toshitaka Browse this author | | Yeh, Syun-Ru Browse this author | | Ishimori, Koichiro Browse this author |
| Issue Date: | 7-Jul-2017 |
| Publisher: | Royal Society of Chemistry |
| Journal Title: | Dalton transactions |
| Volume: | 46 |
| Issue: | 25 |
| Start Page: | 8104 |
| End Page: | 8109 |
| Publisher DOI: | 10.1039/C7DT01562C |
| Abstract: | HutZ is a heme-degrading enzyme in Vibrio cholerae. It converts heme to biliverdin via verdoheme, suggesting that it follows the same reaction mechanism as that of mammalian heme oxygenase. However, none of the key intermediates have been identified. In this study, we applied steady-state and time-resolved UV-vis absorption and resonance Raman spectroscopy to study the reaction of the heme– HutZ complex with H2O2 or ascorbic acid. We characterized three intermediates: oxyferrous heme, meso-hydroxyheme, and verdoheme complexes. Our data support the view that HutZ degrades heme in a manner similar to mammalian heme oxygenase, despite their low sequence and structural homology. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/70925 |
| Appears in Collections: | 理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 内田 毅
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