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Reaction intermediates in the heme degradation reaction by HutZ from Vibrio cholerae

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Title: Reaction intermediates in the heme degradation reaction by HutZ from Vibrio cholerae
Authors: Uchida, Takeshi Browse this author →KAKEN DB
Sekine, Yukari Browse this author
Dojun, Nobuhiko Browse this author
Lewis-Ballester, Ariel Browse this author
Ishigami, Izumi Browse this author
Matsui, Toshitaka Browse this author
Yeh, Syun-Ru Browse this author
Ishimori, Koichiro Browse this author
Issue Date: 7-Jul-2017
Publisher: Royal Society of Chemistry
Journal Title: Dalton transactions
Volume: 46
Issue: 25
Start Page: 8104
End Page: 8109
Publisher DOI: 10.1039/C7DT01562C
Abstract: HutZ is a heme-degrading enzyme in Vibrio cholerae. It converts heme to biliverdin via verdoheme, suggesting that it follows the same reaction mechanism as that of mammalian heme oxygenase. However, none of the key intermediates have been identified. In this study, we applied steady-state and time-resolved UV-vis absorption and resonance Raman spectroscopy to study the reaction of the heme– HutZ complex with H2O2 or ascorbic acid. We characterized three intermediates: oxyferrous heme, meso-hydroxyheme, and verdoheme complexes. Our data support the view that HutZ degrades heme in a manner similar to mammalian heme oxygenase, despite their low sequence and structural homology.
Type: article (author version)
Appears in Collections:理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 内田 毅

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