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Effects of organic matter in livestock manure digester liquid on microbial community structure and in situ activity of anammox granules

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Title: Effects of organic matter in livestock manure digester liquid on microbial community structure and in situ activity of anammox granules
Authors: Kindaichi, Tomonori Browse this author
Awata, Takanori Browse this author
Mugimoto, Yuichiro Browse this author
Rathnayake, Rathnayake M. L. D. Browse this author
Kasahara, Shinsuke Browse this author
Satoh, Hisashi Browse this author →KAKEN DB
Keywords: Anaerobic ammonium oxidation (anammox)
Digester liquid
Fluorescence in situ hybridization (FISH)
Phylogenetic analysis
Issue Date: Sep-2016
Publisher: Elsevier
Journal Title: Chemosphere
Volume: 159
Start Page: 300
End Page: 307
Publisher DOI: 10.1016/j.chemosphere.2016.06.018
PMID: 27314631
Abstract: Anaerobic ammonium oxidation (anammox) is a promising process for NH4+-rich wastewaters such as anaerobic digester liquids. In the present study, we investigated various properties of an up-flow column reactor containing anammox granules and fed with a real digester liquid at four different concentrations (Phases 1 to 4). The efficiencies of NH4+ and NO2- removal decreased by up to 32% and 42%, respectively, in the digester-liquid-fed reactor (reactor-DL). When the performance of reactor-DL deteriorated, the community structure, spatial distribution, and in situ anammox activity in the two reactors were further investigated using 16S rRNA gene-based phylogenetic analysis, fluorescence in situ hybridization (FISH), and microelectrode measurements. The phylogenetic analysis and FISH results showed that non-anammox bacteria were predominant in the granule outer layers in reactor-DL, whereas anammox bacteria still dominated the granule interiors. Microelectrode measurements showed clear evidence of NH4+ oxidation activity in the interiors of granules from reactor-DL. Batch experiments using anammox granules at different acetate concentrations indicated that concentrations up to 50 mM had no effects on the anammox activity, whereas inorganic carbon uptake decreased in the presence of acetate. The present study clearly shows that the anammox activity and anammox bacterial density in the granules were maintained after feeding the digester liquid to the reactor for 140 days.
Rights: © 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license
Type: article (author version)
Appears in Collections:工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 佐藤 久

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