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Different contributions of nonmuscle myosin IIA and IIB to the organization of stress fiber subtypes in fibroblasts

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Title: Different contributions of nonmuscle myosin IIA and IIB to the organization of stress fiber subtypes in fibroblasts
Authors: Kuragano, Masahiro Browse this author
Uyeda, Taro Q. P. Browse this author
Kamijo, Keiju Browse this author
Murakami, Yota Browse this author →KAKEN DB
Takahashi, Masayuki Browse this author →KAKEN DB
Issue Date: 15-Apr-2018
Publisher: American Society for Cell Biology
Journal Title: Molecular biology of the cell
Volume: 29
Issue: 8
Start Page: 911
End Page: 922
Publisher DOI: 10.1091/mbc.E17-04-0215
Abstract: Stress fibers (SFs) are contractile, force-generating bundled structures that can be classified into three subtypes, namely ventral SFs (vSFs), transverse arcs (TAs), and dorsal SFs. Nonmuscle myosin II (NMII) is the main component of SFs. This study examined the roles of the NMII isoforms NMIIA and NMIIB in the organization of each SF subtype in immortalized fibroblasts. Knockdown (KD) of NMIIA (a major isoform) resulted in loss of TAs from the lamella and caused the lamella to lose its flattened shape. Exogenous expression of NMIIB rescued this defect in TA formation. However, the TAs that formed on exogenous NMIIB expression in NMIIA-KD cells and the remaining TAs in NMIIB-KD cells, which mainly consisted of NMIIB and NMIIA, respectively, failed to rescue the defect in lamellar flattening. These results indicate that both isoforms are required for the proper function of TAs in lamellar flattening. KD of NMIIB resulted in loss of vSFs from the central region of the cell body, and this defect was not rescued by exogenous expression of NMIIA, indicating that NMIIA cannot replace the function of NMIIB in vSF formation. Moreover, we raised the possibility that actin filaments in vSFs are in a stretched conformation.
Type: article
Appears in Collections:理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 高橋 正行

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