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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Health Sciences / Faculty of Health Sciences >
Peer-reviewed Journal Articles, etc >
Profiling of cardiolipins and their hydroperoxides in HepG2 cells by LC/MS
| Title: | Profiling of cardiolipins and their hydroperoxides in HepG2 cells by LC/MS |
| Authors: | Chen, Zhen Browse this author | | Wu, Yue Browse this author | | Ma, Yi-Shing Browse this author | | Kobayashi, Yuu Browse this author | | Zhao, Yao-Yao Browse this author | | Miura, Yusuke Browse this author | | Chiba, Hitoshi Browse this author →KAKEN DB | | Hui, Shu-Ping Browse this author →KAKEN DB |
| Keywords: | Cardiolipin | | Lipid hydroperoxides | | CL-OOH | | Molecular species | | Mitochondria | | LC-HR-MS/MS |
| Issue Date: | Sep-2017 |
| Publisher: | Springer |
| Journal Title: | Analytical and bioanalytical chemistry |
| Volume: | 409 |
| Issue: | 24 |
| Start Page: | 5735 |
| End Page: | 5745 |
| Publisher DOI: | 10.1007/s00216-017-0515-3 |
| PMID: | 28762068 |
| Abstract: | Cardiolipin (CL) exists as crucial functional phospholipid in mitochondria. The oxidation of CL is concerned with mitochondrial dysfunction and various diseases. As main oxidation products, CL hydroperoxide (CL-OOH) plays a key role in intermediating oxidative reaction. Thus, direct analysis of CL-OOH is of great interest. In the present study, CL and CL-OOH profiles were analyzed in oxidized HepG2 cell lipid via HPLC-Orbitrap MS/MS. Furthermore, the contents of individual molecular species were compared between intact and AAPH-oxidized HepG2 cells. In total, 46 CL and 18 CL-OOH were identified from oxidized cell lipids, while 21 CL and 9 CL-OOH were detected in AAPH-treated cells. Most CL depleted significantly after AAPH inducement, with percentages varying from 8.3% (CL70:7) to 73.7% (CL72:4), depending on fatty acyl composition. While almost all the CL-OOH remarkably increased, among them 68:6-, 72:6-, and 72:7-OOHs were only detected in AAPH-treated cells. CL68:5- and CL68:4-OOH were the most abundant species, while CL70:5-OOH among all the species expressed the highest oxidation percentage of the corresponding CL. Our results showed practical separation, identification, and semi-quantitation of CL-OOH species, which could contribute to approaches to lipidomic analysis of CL and CL-OOH, as well as tracing biomarkers in mitochondrial oxidative stress diagnosis. |
| Rights: | The final publication is available at link.springer.com via http://dx.doi.org/10.1007/s00216-017-0515-3. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/71563 |
| Appears in Collections: | 保健科学院・保健科学研究院 (Graduate School of Health Sciences / Faculty of Health Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 陳 震
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