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Profiling of cardiolipins and their hydroperoxides in HepG2 cells by LC/MS

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Title: Profiling of cardiolipins and their hydroperoxides in HepG2 cells by LC/MS
Authors: Chen, Zhen Browse this author
Wu, Yue Browse this author
Ma, Yi-Shing Browse this author
Kobayashi, Yuu Browse this author
Zhao, Yao-Yao Browse this author
Miura, Yusuke Browse this author
Chiba, Hitoshi Browse this author →KAKEN DB
Hui, Shu-Ping Browse this author →KAKEN DB
Keywords: Cardiolipin
Lipid hydroperoxides
Molecular species
Issue Date: Sep-2017
Publisher: Springer
Journal Title: Analytical and bioanalytical chemistry
Volume: 409
Issue: 24
Start Page: 5735
End Page: 5745
Publisher DOI: 10.1007/s00216-017-0515-3
PMID: 28762068
Abstract: Cardiolipin (CL) exists as crucial functional phospholipid in mitochondria. The oxidation of CL is concerned with mitochondrial dysfunction and various diseases. As main oxidation products, CL hydroperoxide (CL-OOH) plays a key role in intermediating oxidative reaction. Thus, direct analysis of CL-OOH is of great interest. In the present study, CL and CL-OOH profiles were analyzed in oxidized HepG2 cell lipid via HPLC-Orbitrap MS/MS. Furthermore, the contents of individual molecular species were compared between intact and AAPH-oxidized HepG2 cells. In total, 46 CL and 18 CL-OOH were identified from oxidized cell lipids, while 21 CL and 9 CL-OOH were detected in AAPH-treated cells. Most CL depleted significantly after AAPH inducement, with percentages varying from 8.3% (CL70:7) to 73.7% (CL72:4), depending on fatty acyl composition. While almost all the CL-OOH remarkably increased, among them 68:6-, 72:6-, and 72:7-OOHs were only detected in AAPH-treated cells. CL68:5- and CL68:4-OOH were the most abundant species, while CL70:5-OOH among all the species expressed the highest oxidation percentage of the corresponding CL. Our results showed practical separation, identification, and semi-quantitation of CL-OOH species, which could contribute to approaches to lipidomic analysis of CL and CL-OOH, as well as tracing biomarkers in mitochondrial oxidative stress diagnosis.
Rights: The final publication is available at via
Type: article (author version)
Appears in Collections:保健科学院・保健科学研究院 (Graduate School of Health Sciences / Faculty of Health Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 陳 震

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