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An Easy-to-Use Polystyrene Microchip-based Cell Culture System

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Title: An Easy-to-Use Polystyrene Microchip-based Cell Culture System
Authors: TAZAWA, Hidekatsu Browse this author
SUNAOSHI, Shohei Browse this author
TOKESHI, Manabu Browse this author →KAKEN DB
KITAMORI, Takehiko Browse this author
OHTANI-KANEKO, Ritsuko Browse this author
Keywords: Microchip
cell assay
bubble trap
endothelial cell
cell-response monitoring
Issue Date: 10-Mar-2016
Publisher: 日本分析化学会
Journal Title: Analytical Sciences
Volume: 32
Issue: 3
Start Page: 349
End Page: 353
Publisher DOI: 10.2116/analsci.32.349
Abstract: In this study, we developed an integrated, low-cost microfluidic cell culture system that is easy to use. This system consists of a disposable polystyrene microchip, a polytetrafluoroethylene valve, an air bubble trap, and an indium tin oxide temperature controller. Valve pressure resistance was validated with a manometer to be 3 MPa. The trap protected against bubble contamination. The temperature controller enabled the culture of Macaca mulatta RF/6A 135 vascular endothelial cells, which are difficult to culture in glass microchips, without a CO2 incubator. We determined the optimal coating conditions for these cells and were able to achieve stable, confluent culture within 1 week. This practical system is suitable for low-cost screening and has potential applications as circulatory cell culture systems and research platforms in cell biology.
Type: article
Appears in Collections:工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 渡慶次 学

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