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An Easy-to-Use Polystyrene Microchip-based Cell Culture System
Title: | An Easy-to-Use Polystyrene Microchip-based Cell Culture System |
Authors: | TAZAWA, Hidekatsu Browse this author | SUNAOSHI, Shohei Browse this author | TOKESHI, Manabu Browse this author →KAKEN DB | KITAMORI, Takehiko Browse this author | OHTANI-KANEKO, Ritsuko Browse this author |
Keywords: | Microchip | microfluidics | cell assay | bubble trap | endothelial cell | cell-response monitoring |
Issue Date: | 10-Mar-2016 |
Publisher: | 日本分析化学会 |
Journal Title: | Analytical Sciences |
Volume: | 32 |
Issue: | 3 |
Start Page: | 349 |
End Page: | 353 |
Publisher DOI: | 10.2116/analsci.32.349 |
Abstract: | In this study, we developed an integrated, low-cost microfluidic cell culture system that is easy to use. This system consists of a disposable polystyrene microchip, a polytetrafluoroethylene valve, an air bubble trap, and an indium tin oxide temperature controller. Valve pressure resistance was validated with a manometer to be 3 MPa. The trap protected against bubble contamination. The temperature controller enabled the culture of Macaca mulatta RF/6A 135 vascular endothelial cells, which are difficult to culture in glass microchips, without a CO2 incubator. We determined the optimal coating conditions for these cells and were able to achieve stable, confluent culture within 1 week. This practical system is suitable for low-cost screening and has potential applications as circulatory cell culture systems and research platforms in cell biology. |
Type: | article |
URI: | http://hdl.handle.net/2115/71660 |
Appears in Collections: | 工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 渡慶次 学
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