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Parallel Real-Time PCR on a Chip for Genetic Tug-of-War (gTOW) Method

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Title: Parallel Real-Time PCR on a Chip for Genetic Tug-of-War (gTOW) Method
Authors: NAITO, Toyohiro Browse this author
YATSUHASHI, Ai Browse this author
KAJI, Noritada Browse this author
ANDO, Taeko Browse this author
SATO, Kazuo Browse this author
MORIYA, Hisao Browse this author
KITANO, Hiroaki Browse this author
YASUI, Takao Browse this author
TOKESHI, Manabu Browse this author →KAKEN DB
BABA, Yoshinobu Browse this author
Keywords: microchip-based
Issue Date: 10-Mar-2013
Publisher: 日本分析化学会
Journal Title: Analytical Sciences
Volume: 29
Issue: 3
Start Page: 367
End Page: 371
Publisher DOI: 10.2116/analsci.29.367
Abstract: A microchip-based real-time polymerase chain reaction (PCR) device has been developed for the genetic tug-of-war (gTOW) method that provides quantitative data for research on biorobustness and systems biology. The device was constructed of a silicon glass chip, a temperature controlling Peltier element, and a microscope. A parallel real-time amplification process of target genes on the plasmids and the housekeeping genes in a model eukaryote Saccharomyces cerevisiae were detected simultaneously, and the copy number of the target genes were estimated. The device provides unique quantitative data that can be used to augment understanding of the system-level properties of living cells.
Type: article
Appears in Collections:工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 渡慶次 学

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