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Parallel Real-Time PCR on a Chip for Genetic Tug-of-War (gTOW) Method
| Title: | Parallel Real-Time PCR on a Chip for Genetic Tug-of-War (gTOW) Method |
| Authors: | NAITO, Toyohiro Browse this author | | YATSUHASHI, Ai Browse this author | | KAJI, Noritada Browse this author | | ANDO, Taeko Browse this author | | SATO, Kazuo Browse this author | | MORIYA, Hisao Browse this author | | KITANO, Hiroaki Browse this author | | YASUI, Takao Browse this author | | TOKESHI, Manabu Browse this author →KAKEN DB | | BABA, Yoshinobu Browse this author |
| Keywords: | microchip-based |
| Issue Date: | 10-Mar-2013 |
| Publisher: | 日本分析化学会 |
| Journal Title: | Analytical Sciences |
| Volume: | 29 |
| Issue: | 3 |
| Start Page: | 367 |
| End Page: | 371 |
| Publisher DOI: | 10.2116/analsci.29.367 |
| Abstract: | A microchip-based real-time polymerase chain reaction (PCR) device has been developed for the genetic tug-of-war (gTOW) method that provides quantitative data for research on biorobustness and systems biology. The device was constructed of a silicon glass chip, a temperature controlling Peltier element, and a microscope. A parallel real-time amplification process of target genes on the plasmids and the housekeeping genes in a model eukaryote Saccharomyces cerevisiae were detected simultaneously, and the copy number of the target genes were estimated. The device provides unique quantitative data that can be used to augment understanding of the system-level properties of living cells. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/71662 |
| Appears in Collections: | 工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 渡慶次 学
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