Microchip-based Homogeneous Immunoassay Using a Cloned Enzyme Donor


Hokkaido University \| Library \| HUSCAP	Advanced Search		言語

	Home
	About HUSCAP
	Open Access Policy

	Browse by Author

Browse
	Communities & Collections

	Scholarly Journals
	Theses
	Doctoral Dissertations Listed by Graduate Schools
	Conference Procs.
	Events

	HUSCAP Senior (in Japanese)

	Societies

	Downloads (country)

For university staff
	How to post your papers to HUSCAP
	Publication of theses
	Helpline about theses publication

Open Archives Compliant

You can search our collection also at:
	Google
	Google Scholar
	CiNii
	IRDB
	OAIster
	NDLTD

Files in This Item:

Anal.sci.25-149.pdf

256.77 kB

PDF

Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/71664

Title:	Microchip-based Homogeneous Immunoassay Using a Cloned Enzyme Donor
Authors:	TACHI, Tomoya Browse this author
	KAJI, Noritada Browse this author
	TOKESHI, Manabu Browse this author →KAKEN DB
	BABA, Yoshinobu Browse this author
Keywords:	cloned enzyme donor immunoassay (CEDIA)
Issue Date:	10-Feb-2009
Publisher:	日本分析化学会
Journal Title:	Analytical Sciences
Volume:	25
Issue:	2
Start Page:	149
End Page:	151
Publisher DOI:	10.2116/analsci.25.149
Abstract:	We have realized a cloned enzyme donor immunoassay (CEDIA) on a microchip in 96 s. CEDIA is a homogeneous immunoassay, based on the bacterial enzyme β-galactosidase, which was genetically engineered into two inactive fragments: an enzyme donor and an enzyme acceptor. A model analyte was theophylline, and the detectable concentration range was from 0 to 40 µg mL-1. Our CEDIA using a microfluidic device was very simple and rapid, unlike microchip-based heterogeneous immunoassays and CEDIA on a well-type microchip.
Type:	article
URI:	http://hdl.handle.net/2115/71664
Appears in Collections:	工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 渡慶次学

OAI-PMH ( junii2 , jpcoar_1.0 )

- Hokkaido University