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Microchip-based Homogeneous Immunoassay Using a Cloned Enzyme Donor

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Title: Microchip-based Homogeneous Immunoassay Using a Cloned Enzyme Donor
Authors: TACHI, Tomoya Browse this author
KAJI, Noritada Browse this author
TOKESHI, Manabu Browse this author →KAKEN DB
BABA, Yoshinobu Browse this author
Keywords: cloned enzyme donor immunoassay (CEDIA)
Issue Date: 10-Feb-2009
Publisher: 日本分析化学会
Journal Title: Analytical Sciences
Volume: 25
Issue: 2
Start Page: 149
End Page: 151
Publisher DOI: 10.2116/analsci.25.149
Abstract: We have realized a cloned enzyme donor immunoassay (CEDIA) on a microchip in 96 s. CEDIA is a homogeneous immunoassay, based on the bacterial enzyme β-galactosidase, which was genetically engineered into two inactive fragments: an enzyme donor and an enzyme acceptor. A model analyte was theophylline, and the detectable concentration range was from 0 to 40 µg mL-1. Our CEDIA using a microfluidic device was very simple and rapid, unlike microchip-based heterogeneous immunoassays and CEDIA on a well-type microchip.
Type: article
Appears in Collections:工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 渡慶次 学

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