HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Engineering / Faculty of Engineering >
Peer-reviewed Journal Articles, etc >

Microchip Electrophoresis for Specific Gene Detection of the Pathogenic Bacteria V. cholerae by Circle-to-Circle Amplification

Files in This Item:
Anal.sci.24-327.pdf97.99 kBPDFView/Open
Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/71665

Title: Microchip Electrophoresis for Specific Gene Detection of the Pathogenic Bacteria V. cholerae by Circle-to-Circle Amplification
Authors: MAHMOUDIAN, Laili Browse this author
MELIN, Jonas Browse this author
MOHAMADI, Mohamad Reza Browse this author
YAMADA, Keiko Browse this author
OHTA, Michio Browse this author
KAJI, Noritada Browse this author
TOKESHI, Manabu Browse this author →KAKEN DB
NILSSON, Mats Browse this author
BABA, Yoshinobu Browse this author
Keywords: Microchip Electrophoresis
Issue Date: 10-Mar-2008
Publisher: 日本分析化学会
Journal Title: Analytical Sciences
Volume: 24
Issue: 3
Start Page: 327
End Page: 332
Publisher DOI: 10.2116/analsci.24.327
Abstract: We have developed a new method for a fast and precise analysis of circle-to-circle amplification (C2CA) product for specific gene detection by microchip electrophoresis. In this method, we have added a new enzymatic step to the C2CA reaction, which could be carried out isothermally at 37°C. Compared to the original single-stranded DNA, the double-stranded DNA that is produced by this enzymatic reaction is more reliable for analysis by microchip electrophoresis. C2CA product was detected within 55 s with high reproducibility by this method which was successfully applied to the detection of 10-ng genomic DNA of the pathogenic bacteria Vibrio. cholerae within 110 s. Purification was found to be an indispensable step for the analysis of the C2CA product of genomic DNA samples.
Type: article
URI: http://hdl.handle.net/2115/71665
Appears in Collections:工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 渡慶次 学

Export metadata:

OAI-PMH ( junii2 , jpcoar )

MathJax is now OFF:


 

 - Hokkaido University