|
Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Engineering / Faculty of Engineering >
Peer-reviewed Journal Articles, etc >
Microchip Electrophoresis for Specific Gene Detection of the Pathogenic Bacteria V. cholerae by Circle-to-Circle Amplification
| Title: | Microchip Electrophoresis for Specific Gene Detection of the Pathogenic Bacteria V. cholerae by Circle-to-Circle Amplification |
| Authors: | MAHMOUDIAN, Laili Browse this author | | MELIN, Jonas Browse this author | | MOHAMADI, Mohamad Reza Browse this author | | YAMADA, Keiko Browse this author | | OHTA, Michio Browse this author | | KAJI, Noritada Browse this author | | TOKESHI, Manabu Browse this author →KAKEN DB | | NILSSON, Mats Browse this author | | BABA, Yoshinobu Browse this author |
| Keywords: | Microchip Electrophoresis |
| Issue Date: | 10-Mar-2008 |
| Publisher: | 日本分析化学会 |
| Journal Title: | Analytical Sciences |
| Volume: | 24 |
| Issue: | 3 |
| Start Page: | 327 |
| End Page: | 332 |
| Publisher DOI: | 10.2116/analsci.24.327 |
| Abstract: | We have developed a new method for a fast and precise analysis of circle-to-circle amplification (C2CA) product for specific gene detection by microchip electrophoresis. In this method, we have added a new enzymatic step to the C2CA reaction, which could be carried out isothermally at 37°C. Compared to the original single-stranded DNA, the double-stranded DNA that is produced by this enzymatic reaction is more reliable for analysis by microchip electrophoresis. C2CA product was detected within 55 s with high reproducibility by this method which was successfully applied to the detection of 10-ng genomic DNA of the pathogenic bacteria Vibrio. cholerae within 110 s. Purification was found to be an indispensable step for the analysis of the C2CA product of genomic DNA samples. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/71665 |
| Appears in Collections: | 工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
Submitter: 渡慶次 学
|
