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Poly(methylmethacrylate) Microchip Electrophoresis of Proteins Using Linear-poly(acrylamide) Solutions as Separation Matrix

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Title: Poly(methylmethacrylate) Microchip Electrophoresis of Proteins Using Linear-poly(acrylamide) Solutions as Separation Matrix
Authors: OKADA, Hiroki Browse this author
KAJI, Noritada Browse this author
TOKESHI, Manabu Browse this author →KAKEN DB
BABA, Yoshinobu Browse this author
Keywords: Poly(methylmethacrylate) (PMMA)
microchip electrophoresis
sodium dodecyl sulfate-protein complexes (SDS-PC)
Issue Date: 10-Mar-2008
Publisher: 日本分析化学会
Journal Title: Analytical Sciences
Volume: 24
Issue: 3
Start Page: 321
End Page: 325
Publisher DOI: 10.2116/analsci.24.321
Abstract: Poly(methylmethacrylate) (PMMA) microchip electrophoresis of sodium dodecyl sulfate-protein complexes (SDS-PC) using linear-poly(acrylamide) (L-PA) as a separation matrix was investigated. Prior to electrophoresis, channel walls of PMMA were modified with methylcellulose (MC) to prevent adsorption between channel walls and SDS-PC. Size-based protein separation (SBPS) was successfully performed using the MC-coated microchips with Ferguson plot-fittings. The entangled L-PA solution provided high resolution of peaks of SDS-PC when the concentration of L-PA was increased. Some investigations into the separation mechanism, such as the plot of the logarithm of mobility of each SDS-PC versus the logarithm of the molecular weight of the complex exhibiting linear behavior, indicated that the separation mechanism was dependent on mass discrimination, in accordance with Ogston model.
Type: article
Appears in Collections:工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 渡慶次 学

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