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Poly(methylmethacrylate) Microchip Electrophoresis of Proteins Using Linear-poly(acrylamide) Solutions as Separation Matrix
| Title: | Poly(methylmethacrylate) Microchip Electrophoresis of Proteins Using Linear-poly(acrylamide) Solutions as Separation Matrix |
| Authors: | OKADA, Hiroki Browse this author | | KAJI, Noritada Browse this author | | TOKESHI, Manabu Browse this author →KAKEN DB | | BABA, Yoshinobu Browse this author |
| Keywords: | Poly(methylmethacrylate) (PMMA) | | microchip electrophoresis | | sodium dodecyl sulfate-protein complexes (SDS-PC) |
| Issue Date: | 10-Mar-2008 |
| Publisher: | 日本分析化学会 |
| Journal Title: | Analytical Sciences |
| Volume: | 24 |
| Issue: | 3 |
| Start Page: | 321 |
| End Page: | 325 |
| Publisher DOI: | 10.2116/analsci.24.321 |
| Abstract: | Poly(methylmethacrylate) (PMMA) microchip electrophoresis of sodium dodecyl sulfate-protein complexes (SDS-PC) using linear-poly(acrylamide) (L-PA) as a separation matrix was investigated. Prior to electrophoresis, channel walls of PMMA were modified with methylcellulose (MC) to prevent adsorption between channel walls and SDS-PC. Size-based protein separation (SBPS) was successfully performed using the MC-coated microchips with Ferguson plot-fittings. The entangled L-PA solution provided high resolution of peaks of SDS-PC when the concentration of L-PA was increased. Some investigations into the separation mechanism, such as the plot of the logarithm of mobility of each SDS-PC versus the logarithm of the molecular weight of the complex exhibiting linear behavior, indicated that the separation mechanism was dependent on mass discrimination, in accordance with Ogston model. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/71666 |
| Appears in Collections: | 工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 渡慶次 学
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