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Optimization of an Interface Chip for Coupling Capillary Electrophoresis with Thermal Lens Microscopic Detection

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Title: Optimization of an Interface Chip for Coupling Capillary Electrophoresis with Thermal Lens Microscopic Detection
Authors: UCHIYAMA, Kenji Browse this author
TOKESHI, Manabu Browse this author →KAKEN DB
KIKUTANI, Yoshikuni Browse this author
HATTORI, Akihiko Browse this author
KITAMORI, Takehiko Browse this author
Keywords: capillary-to-microchip connection
Issue Date: Jan-2005
Publisher: 日本分析化学会
Journal Title: Analytical Sciences
Volume: 21
Issue: 1
Start Page: 49
End Page: 52
Publisher DOI: 10.2116/analsci.21.49
Abstract: This paper presents a capillary-to-microchip connection, which can be used as an interface for coupling capillary electrophoresis (CE) with a thermal lens microscope (TLM). It is difficult to directly apply TLM to samples in a capillary with a curved surface, and such an interface chip at the end of a CE separation column is needed for reliable TLM measurements. The dependence of the TLM signal intensity on the TLM detection point in the interface chip and the dependence of the theoretical plate number of CE separation on the channel dimensions of the interface chip were investigated and optimized with a mixture of 4-dimethylaminoazobenzene-4′-sulfonyl(DABSYL)-derivatized amino acids (glycine, alanine, methionine, and proline) as a model sample. By using an optimized interface chip, theoretical plate numbers of DABSYL-glycine, -methionine, -alanine, and -proline were obtained to be 104000, 95000, 104000, and 95000, respectively.
Type: article
Appears in Collections:工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 渡慶次 学

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