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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Engineering / Faculty of Engineering >
Peer-reviewed Journal Articles, etc >
Sucrose supplementation suppressed the growth inhibition in polyhydroxyalkanoate-producing plants
| Title: | Sucrose supplementation suppressed the growth inhibition in polyhydroxyalkanoate-producing plants |
| Authors: | Yoshizumi, Takeshi Browse this author | | Yamada, Miwa Browse this author | | Higuchi-Takeuchi, Mieko Browse this author | | Matsumoto, Ken′ichiro Browse this author →KAKEN DB | | Taguchi, Seiichi Browse this author | | Matsui, Minami Browse this author | | Numata, Keiji Browse this author |
| Keywords: | biosynthesis | | cytotoxicity | | metabolic pathway | | polyhydroxyalkanoate |
| Issue Date: | 31-Mar-2017 |
| Publisher: | 日本植物細胞分子生物学会 |
| Journal Title: | Plant Biotechnology |
| Volume: | 34 |
| Issue: | 1 |
| Start Page: | 39 |
| End Page: | 43 |
| Publisher DOI: | 10.5511/plantbiotechnology.16.1121a |
| Abstract: | Polyhydroxyalkanoate (PHA) is a thermoplastic polymer with several advantageous properties, including biomass origin, biocompatibility, and biodegradability. PHA is synthesized in transgenic plants harboring 3 enzymatic genes: phaA, phaB, and phaC (collectively referred to as phaABC). PHA-producing plants exhibit severe growth inhibition that leads to extremely low PHA accumulation when these enzymes are localized in the cytosol. This growth inhibition could be attributed to the deleterious effects of the PHA biosynthetic pathway on endogenous essential metabolites or to PHA cytotoxicity itself. We performed precise morphological observations of phaABC-overexpressing Arabidopsis (ABC-ox), which displayed typical growth inhibition. On growth medium without sucrose, ABC-ox exhibited a pale green phenotype, dwarfism, including small cotyledons and true leaves, and short roots. ABC-ox partially recovered from this growth inhibition when the growth medium was supplemented with 1% sucrose. This recovery was reversed after ABC-ox grown on 1% sucrose medium was transferred to soil. ABC-ox grown on 1% sucrose medium not only demonstrated recovery from growth inhibition but were also the only examined plants with PHA accumulation, suggesting that growth inhibition was not caused by PHA cytotoxicity but rather by a lack of essential metabolites. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/71679 |
| Appears in Collections: | 工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 松本 謙一郎
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