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マイクロ抽出場によるヒト腫瘍細胞由来のマイクロRNA抽出法
| Title: | マイクロ抽出場によるヒト腫瘍細胞由来のマイクロRNA抽出法 |
| Other Titles: | Development of a Micro RNA Extraction Chip from Human Tumor Cells |
| Authors: | 岡本, 行広1 Browse this author | | 日比野, 理人2 Browse this author | | 加地, 範匡3 Browse this author | | 渡慶次, 学4 Browse this author →KAKEN DB | | 馬場, 嘉信5 Browse this author |
| Authors(alt): | Okamoto, Yukihiro1 | | Hibino, Ayato2 | | Kaji, Noritada3 | | Tokeshi, Manabu4 | | Baba, Yoshinobu5 |
| Keywords: | micro RNA | | microchip | | silica membrane |
| Issue Date: | Jan-2015 |
| Publisher: | 日本分析化学会 |
| Journal Title: | 分析化学 |
| Volume: | 64 |
| Issue: | 1 |
| Start Page: | 9 |
| End Page: | 13 |
| Publisher DOI: | 10.2116/bunsekikagaku.64.9 |
| Abstract: | The importance of micro RNA analysis has been significantly increasing because the role of micro RNA in the human body has been gradually revealed. Despite its importance, the analysis has suffered from several troublesome pretreatments, which hamper any easy analysis. Therefore, an easy and high-throughput pretreatment method has been demanded. In this paper, we focused on the great advantages of microchip pretreatments over conventional manual pretreatments and developed a microchip for micro RNA extraction. To simplify microchip fabrication, we adopted poly(dimethyl siloxane) (PDMS) microchip and a silica membrane, which has rolls in RNA extraction fields. With silica membranes and the adhesion nature of PDMS, we could easily fabricate RNA extraction fields in the microchip. With this microchip, we successfully extracted micro RNA from cancer tumor cells. Though this is a preliminary experiment, and still has many improvement points, our device is expected to be applied for easy and fast micro RNA extraction from biological samples. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/71683 |
| Appears in Collections: | 工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 渡慶次 学
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