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Title: カップ積層型カーボンナノチューブの発光現象とマイクロチップ電気泳動への応用
Other Titles: Luminescence of Cup-Stacked Carbon Nanotubes and Its Application to Microchip Electrophoresis
Authors: 井上, 陽介1 Browse this author
岡本, 行広2 Browse this author
加地, 範匡3 Browse this author
渡慶次, 学4 Browse this author →KAKEN DB
馬場, 嘉信5 Browse this author
Authors(alt): Inoue, Yousuke1
Okamoto, Yukihiro2
Kaji, Noritada3
Tokeshi, Manabu4
Baba, Yoshinobu5
Keywords: microchip electrophoresis
cup-stacked carbon nanotube
indirect detection
Issue Date: Jun-2009
Publisher: 日本分析化学会
Journal Title: 分析化学
Volume: 58
Issue: 6
Start Page: 517
End Page: 521
Publisher DOI: 10.2116/bunsekikagaku.58.517
Abstract: Microchip electrophoresis (MCE) is one of the most suitable methods for biomolecular separation and analysis because of its superior characteristics. In biomolecular separation and analysis, laser induced fluorescent (LIF) detection is frequently employed in MCE and attains high sensitive detection. However, LIF detection requires the labeling of analytes, which contains troublesome procedures and results in decreasing of the separation efficiency. In addition, the labeling reaction at low concentration is significantly difficult, and thus true high sensitive detection is not easily attained and has been desired. Here, we report on the possibility of cup-stacked carbon nanotubes (CSCNTs) for high sensitive label-free detection and high separation efficiency. At first we investigated fluorescence properties of CSCNTs. Our investigation revealed that supernatant solution of CSCNTs after centrifugation had fluorescence at around 500 nm, while CSCNTs suspension did not. The application of CSCNTs fore MCE enabled us to successfully separate and detect DNA without labeling it. Compared to the direct detection method, our method resulted in poor separation efficiency. However, by controlling of the CSCNTs' aspect ratio and the immobilization of functional molecules on the CSCNTs surfaces, we could improve separation efficiency and attain the separation of various samples without labeling samples
従来,マイクロチップ電気泳動でレーザー励起蛍光検出を用いて高感度検出を試みる際に試料の蛍光標識を行う.しかし,蛍光標識は煩雑な操作,標識に伴う分離能の低下及び低濃度試料の蛍光誘導体化効率の低下という問題を有しており,蛍光標識を不要とする高感度検出法の開発が望まれている.著者らは遠心操作によりカップ積層型カーボンナノチューブ(cup stacked carbon nanotubes,CSCNTs)が蛍光を発することを見いだし,泳動液へのCSCNTsの添加によりDNAのマイクロチップ電気泳動分離及び非標識(間接蛍光)検出に成功した.CSCNTsは蛍光を有することに加えて,製造段階でのアスペクト比の調整やCSCNTs表面への機能性分子の固定化が容易であるためにマイクロチップ電気泳動のみならず様々な分野における活用が今後期待される.
Type: article
Appears in Collections:工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 渡慶次 学

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