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デスクトップ熱レンズ顕微鏡の開発
| Title: | デスクトップ熱レンズ顕微鏡の開発 |
| Other Titles: | Development of a desktop-sized thermal lens microscope |
| Authors: | 比企, 伸一郎1 Browse this author | | 渡慶次, 学2 Browse this author →KAKEN DB | | 火原, 彰秀3 Browse this author | | 北森, 武彦4 Browse this author |
| Authors(alt): | Hiki, Shinichiro1 | | Tokeshi, Manabu2 | | Hibara, Akihide3 | | Kitamori, Takehiko4 |
| Keywords: | thermal lens effect | | thermal lens microscope | | ultra high sensitive detection | | non-fluorescent molecule |
| Issue Date: | Aug-2003 |
| Publisher: | 日本分析化学会 |
| Journal Title: | 分析化学 |
| Volume: | 52 |
| Issue: | 8 |
| Start Page: | 569 |
| End Page: | 574 |
| Publisher DOI: | 10.2116/bunsekikagaku.52.569 |
| Abstract: | A desktop-sized thermal lens microscope (DT-TLM) having high sensitivity and wide applicability was developed. The operationality of the DT-TLM compared with that of a big-sized TLM has been remarkably improved by simplifying the optics and optimizing the optical configuration. In order to evaluate the performance, the DT-TLM was applied to the ultrasensitive detection of a non-fluorescent molecule (dye: Sunset Yellow) in water. The concentration dependence of the thermal lens signal (calibration curve) showed good linearity in the range of 1×10－8～1×10－7 M. From the value of twice the standard deviation （2σ）of this calibration curve, the lower limit of the quantitative determination was estimated to be 1.10×10－8 M. The lower limit of detection was estimated as 8.27×10－9 M from the conditions of the signal-to-noise ration S/N=2. These obtained values were almost the same as that of the big-sized TLM. | | 超高感度かつはん用性の高い，デスクトップサイズの熱レンズ顕微鏡（DT-TLM）を開発した．光学系の単純化と最適化によって，DT-TLMの操作性は従来の大型TLMに比較して大きく向上した．非蛍光性の色素分子（Sunset Yellow）水溶液を用いて性能評価したところ，色素分子濃度1×10－8～1×10－7 Mの範囲で良好な直線関係を示した．検量線の標準偏差（2σ）から定量下限値は1.10×10－8 M，S/N比（＝2）から検出下限値は8.27×10－9 Mとそれぞれ見積もられた．これらの結果は大型TLMとほぼ同等の値であった． |
| Type: | article |
| URI: | http://hdl.handle.net/2115/71687 |
| Appears in Collections: | 工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 渡慶次 学
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