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Title: デスクトップ熱レンズ顕微鏡の開発
Other Titles: Development of a desktop-sized thermal lens microscope
Authors: 比企, 伸一郎1 Browse this author
渡慶次, 学2 Browse this author →KAKEN DB
火原, 彰秀3 Browse this author
北森, 武彦4 Browse this author
Authors(alt): Hiki, Shinichiro1
Tokeshi, Manabu2
Hibara, Akihide3
Kitamori, Takehiko4
Keywords: thermal lens effect
thermal lens microscope
ultra high sensitive detection
non-fluorescent molecule
Issue Date: Aug-2003
Publisher: 日本分析化学会
Journal Title: 分析化学
Volume: 52
Issue: 8
Start Page: 569
End Page: 574
Publisher DOI: 10.2116/bunsekikagaku.52.569
Abstract: A desktop-sized thermal lens microscope (DT-TLM) having high sensitivity and wide applicability was developed. The operationality of the DT-TLM compared with that of a big-sized TLM has been remarkably improved by simplifying the optics and optimizing the optical configuration. In order to evaluate the performance, the DT-TLM was applied to the ultrasensitive detection of a non-fluorescent molecule (dye: Sunset Yellow) in water. The concentration dependence of the thermal lens signal (calibration curve) showed good linearity in the range of 1×10-8~1×10-7 M. From the value of twice the standard deviation (2σ)of this calibration curve, the lower limit of the quantitative determination was estimated to be 1.10×10-8 M. The lower limit of detection was estimated as 8.27×10-9 M from the conditions of the signal-to-noise ration S/N=2. These obtained values were almost the same as that of the big-sized TLM.
超高感度かつはん用性の高い,デスクトップサイズの熱レンズ顕微鏡(DT-TLM)を開発した.光学系の単純化と最適化によって,DT-TLMの操作性は従来の大型TLMに比較して大きく向上した.非蛍光性の色素分子(Sunset Yellow)水溶液を用いて性能評価したところ,色素分子濃度1×10-8~1×10-7 Mの範囲で良好な直線関係を示した.検量線の標準偏差(2σ)から定量下限値は1.10×10-8 M,S/N比(=2)から検出下限値は8.27×10-9 Mとそれぞれ見積もられた.これらの結果は大型TLMとほぼ同等の値であった.
Type: article
Appears in Collections:工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 渡慶次 学

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