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Effect of Cationic Surfactants on Enhancement of Firefly Bioluminescence in the Presence of Liposomes

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Title: Effect of Cationic Surfactants on Enhancement of Firefly Bioluminescence in the Presence of Liposomes
Authors: TANI, Hirofumi Browse this author →KAKEN DB
NOTANI, Mizuyo Browse this author
KAMIDATE, Tamio Browse this author
Keywords: Firefly bioluminescence (BL)
Issue Date: 10-Sep-2008
Publisher: 日本分析化学会
Journal Title: Analytical Sciences
Volume: 24
Issue: 9
Start Page: 1111
End Page: 1115
Publisher DOI: 10.2116/analsci.24.1111
Abstract: Firefly bioluminescence (BL) was greatly affected by cationic surfactants coexisting with liposomes containing phosphatidylcholine and cholesterol. In this study, the effects of the type and concentration of cationic surfactants on BL were studied in the presence of the liposomes. Three types of cationic surfactant: benzalkonium chloride (BAC), n-dodecyltrimethylammonium bromide (DTAB), and benzethonium chloride (BZC), were used. As a common effect in these surfactants, BL intensity was increased and then drastically decreased with increasing surfactant concentration. This can be explained by the formation of cationic liposomes as BL enhancers at low concentration of the surfactant, and by the transformation into cationic (mixed) micelles as inhibitors at high concentration. The maximal BL intensity and the concentration for the maximal BL were dependent on the type of the surfactants. To explain the differences in these parameters in the enhanced BL, we determined the distribution coefficient, K, of the surfactants to the liposomal membrane. The result indicated that the surfactant with higher K value gives the maximal BL intensity at lower concentration.
Type: article
Appears in Collections:工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 谷 博文

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