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Effect of Mixing Modes on Chemiluminescent Detection of Epinephrine with Lucigenin by an FIA System Fabricated on a Microchip

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Title: Effect of Mixing Modes on Chemiluminescent Detection of Epinephrine with Lucigenin by an FIA System Fabricated on a Microchip
Authors: KAMIDATE, Tamio Browse this author
KAIDE, Tomoyuki Browse this author
TANI, Hirofumi Browse this author →KAKEN DB
MAKINO, Eiji Browse this author
SHIBATA, Takayuki Browse this author
Keywords: chemiluminescent (CL)
epinephrine (EP)
lucigenin (Luc)
Issue Date: Aug-2001
Publisher: 日本分析化学会
Journal Title: Analytical Sciences
Volume: 17
Issue: 8
Start Page: 951
End Page: 955
Publisher DOI: 10.2116/analsci.17.951
Abstract: The chemiluminescent (CL) detection of epinephrine (EP) with lucigenin (Luc) was performed using a micro flow cell fabricated on a silicon chip. A solution of EP was injected into the Luc carrier stream. The Luc solution containing EP and an alkaline solution were successively poured into the flow cell by a pressure-driven flow system. Two types of flow cells were fabricated for estimating the effect of the mixing modes in the flow cells on the intensity of light emission. In flow cell 1, two streams entered through separate inlet ports and merged to flow adjacently. In flow cell 2, a Luc solution containing EP was split up to 36 partial flows by passage through the nozzles, and was injected into the alkaline solution. The intensity of light emission in flow cell 2 increased markedly compared to that in flow cell 1. The detection limit of 8.0 × 10-7 M for EP in flow cell 2 was a factor of six-times better than that in flow cell 1. The improvement in the sensitivity for EP could be explained in terms of the distortion of laminar flow in flow cell 2.
Type: article
URI: http://hdl.handle.net/2115/71703
Appears in Collections:工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 谷 博文

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