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Enhancement of Firefly Bioluminescence Using Liposomes Containing Cationic Components
| Title: | Enhancement of Firefly Bioluminescence Using Liposomes Containing Cationic Components |
| Authors: | KAMIDATE, Tamio Browse this author | | FUJITA, Taigo Browse this author | | TANI, Hirofumi Browse this author →KAKEN DB | | WATANABE, Hiroto Browse this author |
| Keywords: | Firefly bioluminescence | | adenosine-5'-triphosphate | | liposome | | stearyltrimethylammonium chloride | | stearylamine | | membrane surface potential |
| Issue Date: | Dec-1998 |
| Publisher: | 日本分析化学会 |
| Journal Title: | Analytical Sciences |
| Volume: | 14 |
| Issue: | 6 |
| Start Page: | 1115 |
| End Page: | 1119 |
| Publisher DOI: | 10.2116/analsci.14.1115 |
| Abstract: | The effect of cationic components in liposome on the bioluminescence (BL) intensity from the firefly luciferin-luciferase reaction with ATP was investigated by use of vesicles formed by the extrusion technique (VET). Stearyltrimethyl-ammonium chloride (STAC), stearylamine (SA) and tetracaine (TC) were used as cationic components. The VET containing STAC and SA enhanced the maximum BL intensity. In contrast, a lowering of the maximum BL intensity was observed in the presence of the VET containing TC. The sensitivity for ATP in the presence of the VET containing STAC was improved by a factor of 2 and 10 times compared to that in the presence of the VET containing SA and that in water alone, respectively. The differences in the BL enhancement between cationic components could be explained in terms of different membrane surface potentials of the VET containing these cationc components. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/71705 |
| Appears in Collections: | 工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 谷 博文
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