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Enhancement of Firefly Bioluminescence Using Liposomes Containing Cationic Components

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Title: Enhancement of Firefly Bioluminescence Using Liposomes Containing Cationic Components
Authors: KAMIDATE, Tamio Browse this author
FUJITA, Taigo Browse this author
TANI, Hirofumi Browse this author →KAKEN DB
WATANABE, Hiroto Browse this author
Keywords: Firefly bioluminescence
adenosine-5'-triphosphate
liposome
stearyltrimethylammonium chloride
stearylamine
membrane surface potential
Issue Date: Dec-1998
Publisher: 日本分析化学会
Journal Title: Analytical Sciences
Volume: 14
Issue: 6
Start Page: 1115
End Page: 1119
Publisher DOI: 10.2116/analsci.14.1115
Abstract: The effect of cationic components in liposome on the bioluminescence (BL) intensity from the firefly luciferin-luciferase reaction with ATP was investigated by use of vesicles formed by the extrusion technique (VET). Stearyltrimethyl-ammonium chloride (STAC), stearylamine (SA) and tetracaine (TC) were used as cationic components. The VET containing STAC and SA enhanced the maximum BL intensity. In contrast, a lowering of the maximum BL intensity was observed in the presence of the VET containing TC. The sensitivity for ATP in the presence of the VET containing STAC was improved by a factor of 2 and 10 times compared to that in the presence of the VET containing SA and that in water alone, respectively. The differences in the BL enhancement between cationic components could be explained in terms of different membrane surface potentials of the VET containing these cationc components.
Type: article
URI: http://hdl.handle.net/2115/71705
Appears in Collections:工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 谷 博文

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