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Selective Solubilization of Microsomal Electron-Transfer Proteins with Alkylglucoside

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Title: Selective Solubilization of Microsomal Electron-Transfer Proteins with Alkylglucoside
Authors: TANI, Hirofumi Browse this author →KAKEN DB
SAITOH, Tohru Browse this author
KAMIDATE, Tamio Browse this author
KAMATAKI, Tetsuya Browse this author
WATANABE, Hiroto Browse this author
Keywords: Alkylglucoside
electro-transfer protein
cytochrome b5
cytochrome P450
Issue Date: Oct-1997
Publisher: 日本分析化学会
Journal Title: Analytical Sciences
Volume: 13
Issue: 5
Start Page: 747
End Page: 751
Publisher DOI: 10.2116/analsci.13.747
Abstract: A series of alkylglucosides (AGs) was examined for the solubilization of four microsomal electron-transfer proteins (cytochrome P450 (P450), cytochrome b5 (b5), NADPH-cytochrome P450 reductase (fp2), and NADH-cytochrome b5 reductase (fp1)) in rat liver microsomes. Among the four proteins, b5 and fp2 were completely solubilized, and thus, almost recovered in the supernatant fraction after centrifugation, while P450 and fp1 were in the pellet. In particular, the solubilization yield of P450 was only about 10%. With a high ratio of alkylglucoside to membrane, along with a low ionic strength, a greater selectivity for b5 and fp2 could be obtained. Such high selectivity was not observed by the use of sugar ester, bile salt, and poly(oxyethylene) alkylphenyl ether types of surfactants. After re-solubilization of the pellet with sodium cholate, the supernatant fraction contained P450 free from b5 and the final recovery of P450 was about 40%. Thus, this two-step solubilization provides a simple method for the partial purification of P450 in microsomes.
Type: article
Appears in Collections:工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 谷 博文

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