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Equilibrium Study on Interactions between Proteins and Bile-Salt Micelles by Micellar Electrokinetic Chromatography
| Title: | Equilibrium Study on Interactions between Proteins and Bile-Salt Micelles by Micellar Electrokinetic Chromatography |
| Authors: | SAITOH, Tohru Browse this author | | FUKUDA, Teruyuki Browse this author | | TANI, Hirofumi Browse this author →KAKEN DB | | KAMIDATE, Tamio Browse this author | | WATANABE, Hiroto Browse this author |
| Keywords: | Protein | | bile-salt micelle | | binding constant | | micellar electrokinetic chromatography |
| Issue Date: | 10-Aug-1996 |
| Publisher: | 日本分析化学会 |
| Journal Title: | Analytical Sciences |
| Volume: | 12 |
| Issue: | 4 |
| Start Page: | 569 |
| End Page: | 573 |
| Publisher DOI: | 10.2116/analsci.12.569 |
| Abstract: | Interactions between proteins and bile-salt micelles were evaluated on the basis of the binding constants, which were obtained from the migration of proteins in micellar electrokinetic chromatography. The binding constants, Kb=[Pb]/ ([Pw][B]), where [Pb] is the concentration of a protein binding with a bile-salt, [Pw] free protein concentration, and [B] the concentration of bile salt present in the micelles, were successfully determined from the slope of a linear curve of the capacity factor (k′) of the protein against [B]. The binding constants were almost identical to those obtained by a gel- filtration method. The value of Kb increased with increasing the hydrophobicity of the bile salt or that of the protein. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/71708 |
| Appears in Collections: | 工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 谷 博文
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