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Equilibrium Study on Interactions between Proteins and Bile-Salt Micelles by Micellar Electrokinetic Chromatography

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/71708

Title: Equilibrium Study on Interactions between Proteins and Bile-Salt Micelles by Micellar Electrokinetic Chromatography
Authors: SAITOH, Tohru Browse this author
FUKUDA, Teruyuki Browse this author
TANI, Hirofumi Browse this author →KAKEN DB
KAMIDATE, Tamio Browse this author
WATANABE, Hiroto Browse this author
Keywords: Protein
bile-salt micelle
binding constant
micellar electrokinetic chromatography
Issue Date: 10-Aug-1996
Publisher: 日本分析化学会
Journal Title: Analytical Sciences
Volume: 12
Issue: 4
Start Page: 569
End Page: 573
Publisher DOI: 10.2116/analsci.12.569
Abstract: Interactions between proteins and bile-salt micelles were evaluated on the basis of the binding constants, which were obtained from the migration of proteins in micellar electrokinetic chromatography. The binding constants, Kb=[Pb]/ ([Pw][B]), where [Pb] is the concentration of a protein binding with a bile-salt, [Pw] free protein concentration, and [B] the concentration of bile salt present in the micelles, were successfully determined from the slope of a linear curve of the capacity factor (k′) of the protein against [B]. The binding constants were almost identical to those obtained by a gel- filtration method. The value of Kb increased with increasing the hydrophobicity of the bile salt or that of the protein.
Type: article
URI: http://hdl.handle.net/2115/71708
Appears in Collections:工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 谷 博文

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