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Simple separation of good quality bovine oocytes using a microfluidic device

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Title: Simple separation of good quality bovine oocytes using a microfluidic device
Authors: Iwasaki, Wataru Browse this author
Yamanaka, Kenichi Browse this author
Sugiyama, Daisuke Browse this author
Teshima, Yuki Browse this author
Briones-Nagata, Maria Portia Browse this author
Maeki, Masatoshi Browse this author
Yamashita, Kenichi Browse this author
Takahashi, Masashi Browse this author
Miyazaki, Masaya Browse this author
Issue Date: 24-Sep-2018
Publisher: Nature Publishing Group
Journal Title: Scientific reports
Volume: 8
Start Page: 14273
Publisher DOI: 10.1038/s41598-018-32687-6
Abstract: We fabricated a simple microfluidic device for separation of bovine oocytes based on the oocyte quality to improve the conception rate of in vitro fertilization (IVF) by using good quality oocytes. The microfluidic device separates oocytes based on sedimentation rate differences in a sucrose buffer, which is dependent on oocyte quality. The microfluidic device has a 700 mu m width, 1 mm height, and 10 mm long separation channel. Oocytes were injected from the upper half of the separation channel, and they flowed while sinking. The outlets of the separation channel were divided into upper and lower chambers. Good quality oocytes settled faster than poor quality oocytes in sucrose buffer; therefore, good quality oocytes were collected from the lower outlet. We performed IVF after the microfluidic separation of oocytes. The developmental rate to blastocysts of oocytes collected from the lower outlet was significantly higher than those collected from the upper outlet (36.0% vs. 14.1%). This result was comparable to that in the BCB staining method performed as a comparison method (BCB+ : 35.7%, BCB-: 15.4%). These findings indicate that our microfluidic device could be applied to oocyte separation and contribute to improvement of in vitro embryo production system.
Type: article
Appears in Collections:工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 宮崎 真佐也

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