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Application of 4-Iodophenol-enhanced Luminol Chemiluminescence to Direct Detection of Horseradish Peroxidase Encapsulated in Liposomes

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Title: Application of 4-Iodophenol-enhanced Luminol Chemiluminescence to Direct Detection of Horseradish Peroxidase Encapsulated in Liposomes
Authors: KAMIDATE, Tamio Browse this author
MARUYA, Masumi Browse this author
TANI, Hirofumi Browse this author
ISHIDA, Akihiko Browse this author
Keywords: horseradish peroxidase (HRP)
chemiluminescence (CL)
Issue Date: 10-Sep-2009
Publisher: 日本分析化学会
Journal Title: Analytical Sciences
Volume: 25
Issue: 9
Start Page: 1163
End Page: 1166
Publisher DOI: 10.2116/analsci.25.1163
Abstract: 4-Iodophenol was applied to an enhancer in the direct detection of horseradish peroxidase (HRP) encapsulated in liposomes by using luminol chemiluminescence (CL). Luminol, 4-iodophenol and hydrogen peroxide permeate into the inner phase of liposomes containing HRP, resulting in the progress of 4-iodophenol-enhanced luminol CL catalyzed by HRP in liposomes. The CL intensity observed in liposomes was a factor of 150 greater than that observed in a lipid-free bulk solution. The detection limit in the direct detection of HRP encapsulated in liposomes was sensitive by a factor of 30 compared with that in a lipid-free bulk solution. 4-Iodophenol effectively functioned as an enhancer in HRP-catalyzed luminol CL in liposomes.
Type: article
URI: http://hdl.handle.net/2115/71739
Appears in Collections:工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 石田 晃彦

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