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X-ray Diffraction of Protein Crystal Grown in a Nano-liter Scale Droplet in a Microchannel and Evaluation of Its Applicability
| Title: | X-ray Diffraction of Protein Crystal Grown in a Nano-liter Scale Droplet in a Microchannel and Evaluation of Its Applicability |
| Authors: | MAEKI, Masatoshi Browse this author →KAKEN DB | | YOSHIZUKA, Saori Browse this author | | YAMAGUCHI, Hiroshi Browse this author | | KAWAMOTO, Masahide Browse this author | | YAMASHITA, Kenichi Browse this author | | NAKAMURA, Hiroyuki Browse this author | | MIYAZAKI, Masaya Browse this author | | MAEDA, Hideaki Browse this author |
| Keywords: | in-situ X-ray diffraction | | crystallization to X-ray analysis | | nanodroplet-based crystallization |
| Issue Date: | 10-Jan-2012 |
| Publisher: | 日本分析化学会 |
| Journal Title: | Analytical Sciences |
| Volume: | 28 |
| Issue: | 1 |
| Start Page: | 65 |
| End Page: | 68 |
| Publisher DOI: | 10.2116/analsci.28.65 |
| Abstract: | We describe the technical aspects of the in-situ X-ray diffraction of a protein crystal prepared by a nanodroplet-based crystallization method. We were able to obtain diffraction patterns from a crystal grown in a capillary without any manipulation. Especially in our experimental approach, the crystals that moved to the nanodroplet interface were fixed strongly enough to carry out X-ray diffraction measurements that could be attributed to the high surface tension of the nanodroplet. The crystal was damaged by an indirect action of the X-rays because our in-situ X-ray diffraction measurement was carried out in the liquid phase without freezing the crystal; however, the obtained several diffraction patterns were of sufficiently fine quality for the crystal structure factors to be generated. We consider the technical examination presented in this paper to represent a seamless coupling of crystallization to X-ray analysis |
| Type: | article |
| URI: | http://hdl.handle.net/2115/71745 |
| Appears in Collections: | 工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 真栄城 正寿
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