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An oligodeoxyribonucleotide containing 5-formyl-2′-deoxycytidine (fC) at the CpG site forms a covalent complex with DNA cytosine-5 methyltransferases (DNMTs)

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Title: An oligodeoxyribonucleotide containing 5-formyl-2′-deoxycytidine (fC) at the CpG site forms a covalent complex with DNA cytosine-5 methyltransferases (DNMTs)
Authors: Sato, Kousuke Browse this author →KAKEN DB
Kawamoto, Kyoji Browse this author
Shimamura, Shintaro Browse this author
Ichikawa, Satoshi Browse this author →KAKEN DB
Matsuda, Akira Browse this author →KAKEN DB
Keywords: Epigenetic
Nucleic acids
5-Formyl-2 '-deoxycytidine
Covalent bond
DNA methyltransferase
Issue Date: 15-Nov-2016
Publisher: Elsevier
Journal Title: Bioorganic & Medicinal Chemistry Letters
Volume: 26
Issue: 22
Start Page: 5395
End Page: 5398
Publisher DOI: 10.1016/j.bmcl.2016.10.040
PMID: 27780634
Abstract: 5-Methylcytosine (mC) is known to induce epigenetic changes. Ten-eleven translocation (TET) enzymes produce the further oxidized 5-substituted cytosine derivatives, 5-formylcytosine (fC) and 5-carboxylcytosine (caC). However, their roles are unclear thus far. Here, we synthesized oligodeoxyribonucleotides (ODNs) containing 5-formyl-2'-deoxycytidine and examined their interactions with DNA cytosine-5 methyltransferase (DNMT). We found that the ODN sequence containing fCpG formed a covalent complex with both bacterial and mouse recombinant DNMTs in the absence of any cofactors. The covalent bonding with DNMT suggests that the fCpG sequence in DNA may play a role in epigenetic regulation. (C) 2016 Elsevier Ltd. All rights reserved.
Rights: © 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
http://creativecommons.org/licenses/by-nc-nd/4.0/
Type: article (author version)
URI: http://hdl.handle.net/2115/71972
Appears in Collections:薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 佐藤 浩輔

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