HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine >
Japanese Journal of Veterinary Research >
Volume 66 Number 4 >

Universal nematode detection by degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR) of purified nematode nucleic acids

Files in This Item:
Supplement_Multilocus_EPIC.docxSUPPLEMENTAL DATA172.72 kBMicrosoft Word XMLView/Open
p311-316 Daiji Endoh.pdf458.88 kBPDFView/Open
Please use this identifier to cite or link to this item:http://doi.org/10.14943/jjvr.66.4.311

Title: Universal nematode detection by degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR) of purified nematode nucleic acids
Authors: Yoshino, Tomoo Browse this author
Asakawa, Mitsuhiko Browse this author
Osa, Yuichi Browse this author
Kaneko, Masami Browse this author →KAKEN DB
Endoh, Daiji Browse this author
Keywords: degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR)
inverted repeat
molecular typing PCR
Issue Date: Nov-2018
Publisher: Faculty of Veterinary Medicine, Hokkaido University
Journal Title: Japanese Journal of Veterinary Research
Volume: 66
Issue: 4
Start Page: 311
End Page: 316
Abstract: In this study, we have described a modified degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR) method for molecular typing of nematodes collected from wild birds. To design the DOP-PCR, we selected 50 nonamers as DOP-motifs referring the data from a nematode genome. Inverted repeats of nonamers with a 100-1,500 base interval in the reported nematode genomes on the selection of the 50 nonamers. In these nonamers, 5 nonamers showed to create ladder pattern by DOP-PCR on 9, 6, 1, 2 and 3 species of morphologically identified adult nematodes, respectively. Eleven species of nematodes were distinguishable by combining the results of the two primers. It was suggested that the nematode species could be distinguished by DOP-PCR with a combination of 2 nonamers.
Type: bulletin (article)
URI: http://hdl.handle.net/2115/72025
Appears in Collections:Japanese Journal of Veterinary Research > Volume 66 Number 4

Submitter: 獣医学部図書室

Export metadata:

OAI-PMH ( junii2 , jpcoar )

MathJax is now OFF:


 

 - Hokkaido University